转基因水稻PA110-15基于RPA等温扩增及DNA试纸条联检的品系特异性检测方法  

The Event-Specific Detection of Transgenic Rice PA110-15 Based on Recombinase Polymerase Amplification Coupled with DNA Lateral Flow Strip

作　　者：贾玄 李凯[1] 董美[1] 金芜军[1,2] 李亮 苗朝华[1,2] 刘卫晓 宛煜嵩[1,2] 

机构地区：[1]中国农业科学院生物技术研究所,北京100081 [2]农业部转基因植物用微生物环境安全监督检验测试中心(北京),北京100081

出　　处：《分子植物育种》2017年第12期5210-5215,共6页Molecular Plant Breeding

基　　金：转基因生物新品种培育重大专项项目(2013ZX08012-001;2018ZX08012-001)资助

摘　　要：水稻作为最重要的粮食作物之一,其转基因安全性一直受到各国政府和人民的广泛关注。为了保障和引导公众对转基因水稻的正确而全面的认识,转基因生物安全检测工作必不可少。在目前的转基因检测工作中,核酸扩增技术是最为常用的检测方法。PCR技术是最成熟、应用最广泛的一种核酸扩增技术。然而,PCR技术对温控条件的极度依赖性制约它在田间的现场快速检测。本研究以转基因水稻PA110-15为对象,建立了重组酶聚合酶等温扩增及DNA试纸条联检的品系特异性检测方法,减少了对专用仪器的依赖,为快速现场转基因检测提供了新的解决方案。Rice, as one of the most important grain crops, has been concerned about its genetically modified organisms（GMO） biosafety. In order to protect and guide the public with right and comprehensive understanding of transgenic rice, GMOs detection is necessary. Nowadays, nucleic acid amplification is the most popular detection method. Meanwhile, polymerase chain reaction（PCR） is one of most mature and popular nucleic acid amplification techniques. However, PCR depends on variable temperature too much to use in the field for the on-the-spot detection. Therefore, we established the event-specific detection of transgenic rice PA110-15 based on recombinase polymerase amplification（RPA） coupled with DNA lateral flow strip. The method reduced reliance on dedicated instruments and provided a new solution for rapid on-the-spot GMOs detection in the field.

关 键 词：等温扩增 RPA DNA试纸条 转基因检测 转基因水稻PA110-15 

分 类 号：S511[农业科学—作物学]