雷替曲塞联合X线照射对人喉癌细胞Hep-2的协同作用  

The synergistic effect of raltitrexed combined with X-ray radiation on human laryngeal cancer Hep-2 cells

作　　者：王抒 李轩 乔田奎 

机构地区：[1]复旦大学附属金山医院肿瘤科,上海201508

出　　处：《中国临床医学》2017年第6期879-883,共5页Chinese Journal of Clinical Medicine

基　　金：上海市金山区卫生和计划生育委员会青年项目(JSKJ-KTQN-2014-01)~~

摘　　要：目的:探讨雷替曲塞与X射线对人喉癌细胞Hep-2的协同作用及其潜在机制。方法:CCK-8法检测不同浓度雷替曲塞对Hep-2细胞活力的影响。将Hep-2细胞随机分为对照组、雷替曲塞组、照射组、雷替曲塞+照射组。单击多靶模型绘制细胞存活曲线,检测4组细胞存活情况及放射增敏比(sensitization enhancement ratio,SER);流式细胞仪检测4组Hep-2细胞凋亡情况和周期改变。结果:雷替曲塞能抑制Hep-2细胞活力,且呈剂量依赖性(P<0.05)。单击多靶模型显示,雷替曲塞平均致死剂量下SER为1.272。雷替曲塞能显著增加细胞凋亡和G_2/M期阻滞(P<0.05)。结论:雷替曲塞能协同X线对人喉癌细胞Hep-2的抑制作用,其机制可能与诱导肿瘤细胞凋亡和增加细胞G_2/M期阻滞有关。Objective：To investigate the synergistic effect of raltitrexed combined with radiotherapy on Hep-2 cells and its potential mechanism.Methods：CCK-8 assay was used to determine the cell viability after Hep-2 cells were treated with different concentrations of raltitrexed.Hep-2 cells were randomly divided into the control group,the raltitrexed group,the radiation group,and raltitrexed ＋ radiation group.Cell survival curves were fitted with a single-hit multi-target model,cell viability and sensitization enhancement ratio（SNR）of the 4 groups were detected,and apoptosis and cycle changes of Hep-2 cells in 4 groups were detected by flow cytometry.Results：CCK-8 assay proved that raltitrexed could suppress cell viability in a dose-dependent manner.The single-hit multi-target model showed that the SER of mean death dose（D0）was 1.272.Furthermore,the apoptosis rate and the number of Hep-2 cells at G2/M phase were significantly increased in the group treated with the combination of raltitrexed and X-ray as compared to the group treated with X-ray alone（P〈0.05）.Conclusions：The inhibitory effect of raltitrexed combined with X-ray on human laryngeal cancer Hep-2 cells may be related to the induction of tumor cell apoptosis and the increase of G2/M phase arrest.

关 键 词：雷替曲塞 X线 HEP-2细胞 凋亡 细胞周期 

分 类 号：R739.65[医药卫生—肿瘤]