肝细胞肝癌组织精氨酸酶2和诱导型一氧化氮合酶的表达及与肿瘤血管形成的相关性  

Expressions of arginase-2 and inducible nitric oxide synthase in hepatocellular carcinoma and the relations to angiogenesis

作　　者：肖锋[1] 顾春燕[1] 邵建国[2] 钱铮[1] 陈丽燕[1] 孙艳[1] 

机构地区：[1]南通市第三人民医院病理科,南通226006 [2]南通市第三人民医院消化内科,南通226006

出　　处：《中国临床医学》2017年第6期912-915,共4页Chinese Journal of Clinical Medicine

基　　金：南通市前沿与关键技术创新项目(MS22015087);南通市卫计委青年科研基金(WQ2015034;WQ2016016)~~

摘　　要：目的:探讨精氨酸酶2(arginase-2,Arg-2)和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)在肝细胞肝癌(hepatocellular carcinoma,HCC)中的表达及其与肿瘤血管形成的相关性。方法:采用免疫组化方法检测158例HCC患者手术切除标本中Arg-2、iNOS的表达,抗CD34单克隆抗体显示血管内皮细胞。采用Image-Pro plus 6.2.1图像分析软件测定肿瘤微血管密度(microvessel density,MVD)。结果:158例HCC中,Arg-2、iNOS的阳性表达率分别为73.4%(116/158)、83.5%(132/158)。Arg-2和iNOS在HCC组织中的表达正相关(r=0.474,P=0.000)。116例Arg-2阳性HCC组织的MVD为(283.92±130.69)/0.702mm^2,42例Arg-2阴性HCC组织的MVD为(129.25±51.00)/0.702mm^2,差异有统计学意义(P=0.000);132例iNOS阳性组织的MVD为(267±131.49)/0.702mm^2,26例iNOS阴性HCC组织的MVD为(116±41.85)/0.702mm^2,差异有统计学意义(P=0.000)。结论:Arg-2与iNOS在HCC组织中的表达正相关,且与MVD相关,提示Arg-2与iNOS可能参与调节HCC中微血管的形成。Objective：To analyze the expressions of arginase-2（Arg-2）and inducible nitric oxide synthase（iNOS）protein in hepatocellular carcinoma（HCC）and the relations to angiogenesis.Methods：The expressions of Arg-2 and iNOS in tumor specimens from 158 postoperative patients with HCC were detected by using immunohistochemistry.The endothelial cells were immunostained with anti-CD34 antibody.The microvessel density（MVD）was quantified by Image-Pro plus 6.2.1 image analysis software.Results：In 158 HCC patients,the positive rates of Arg-2 and iNOS were 73.4%（116/158）and83.5%（132/116）,respectively.The expressions of Arg-2 and iNOS in HCC were positively correlated（r=0.474,P=0.000）.The MVD was（283.92±130.69）/0.702 mm^2 in 116 HCC specimens with positive expression of Arg-2,and（129.25±51.00）/0.702 mm2 in 42 HCC specimens with negative expression of Arg-2,and the difference was statistically significant（P=0.000）.The MVD was（267±131.49）/0.702 mm^2 in 132 HCC specimens with positive expression of iNOS,and（116±41.85）/0.702 mm^2 in 26 HCC specimens with negative expression of iNOS,and the difference was statistically significant（P=0.000）.Conclusions：The expressions of Arg-2 and iNOS are positively correlated in HCC tissues,which are both related to MVD.These results suggest that Arg-2 and iNOS may be involved in regulating microvascular angiogenesis in HCC.

关 键 词：精氨酸酶2 诱导型一氧化氮合酶 肝细胞肝癌 微血管密度 

分 类 号：R735.7[医药卫生—肿瘤]