血清结肠癌转移相关基因-1在胃癌患者化疗疗效预测中的效果  

Effect of serum MACC1 on forecasting chemotherapeutic effect of patients with gastric cancer

作　　者：陈丽[1] 王小雨 武文娟[1] 袁一枫[1] 

机构地区：[1]江苏省苏北人民医院肿瘤科,江苏扬州225001 [2]大连医科大学研究生院,辽宁大连116044

出　　处：《实用临床医药杂志》2018年第5期1-5,12,共6页Journal of Clinical Medicine in Practice

基　　金：江苏省苏北人民医院院级基金项目(NO:yzucms201501)

摘　　要：目的探讨血清结肠癌转移相关基因-1(MACC1)对胃癌患者化疗疗效的预测作用。方法 MTT法检测MACC1过表达、低表达的胃癌细胞株BCG-823对5-氟脲嘧啶(5-Fu)敏感性的差异。RT-PCR检测MACC1过表达、低表达的胃癌细胞中MACC1 mRNA表达水平。ELISA法检测MACC1过表达、低表达细胞培养液中MACC1蛋白表达水平。分析晚期胃癌患者化疗前外周血中MACC1表达水平与化疗疗效的关系。结果干扰MACC1后,BCG-823细胞中mRNA表达水平下降,过表达细胞中的mRNA表达水平上升,其趋势与细胞培养液中MACC1蛋白表达水平一致。干扰MACC1后,BCG-823对5-Fu敏感性增高,过表达MACC1后其对5-Fu敏感性降低。晚期胃癌患者MACC1蛋白含量较低的患者化疗疗效好于MACC1含量较高的患者。结论 MACC1能够影响胃癌病人对化疗的敏感性,可以有效预测患者化疗疗效。Objective To explore the effect of serum MACC1 on forecasting chemotherapeu- tic effect of patients with gastric cancer. Methods The difference in sensitivity of BCG-823 to 5- fluorouracil （5-Fu） between gastric cancer cell line BCG-823 with over-expression and low expression of MACC1 was detected by MTT method. The expression of MACC1 mRNA in gastric cancer cells with over-expression and low expression was detected by RT-PCR. ELISA was used to detect the expres- sion of MACC1 protein in MACC1 over-expressed and low expression cells. Relationship between the expression of MACC1 in peripheral blood before chemotherapy and the effect of chemotherapy in pa- tients with advanced gastric cancer was analyzed. Results The mRNA expression level of MACC1 decreased after MACC1 silencing and increased after MACCI over-expression, which were consistent with the tendency of protein expression level. After interfering with MACC1, the sensitivity of BCG- 823 to 5-Fu increased, and the sensitivity to 5-Fu reduced after over-expression of MACC1. The patients with low contents of serum MACC1 showed prior chemotherapeutic effect to those with high con- tents of MACC1. Conclusion MACC1 can be used as a useful biomarker for forecasting the efficacy of chemotherapy in patients with gastric cancer.

关 键 词：MACC1 胃癌 5-氟脲嘧啶 生物标志物 

分 类 号：R735.2[医药卫生—肿瘤]