Runx3和c-Myc蛋白在TGF-β抑制MG-63细胞增殖中作用  

The role of Runx3 and c-Myc protein in the inhibitory effect of TGF-β on MG-63 cells proliferation

作　　者：王宁[1] 朴成哲[1] 孟晓娜[2] 

机构地区：[1]沈阳医学院附属中心医院骨科,辽宁沈阳110024 [2]中国医科大学生物化学与分子生物学教研室,辽宁沈阳110122

出　　处：《解剖科学进展》2018年第1期65-68,共4页Progress of Anatomical Sciences

基　　金：辽宁省教育厅基金(L2014417)

摘　　要：目的探讨Runx3和c-Myc蛋白在骨肉瘤组织中的表达及其在MG-63细胞增殖过程中的作用。方法采用Western blot技术检测骨肉瘤组织和骨良性病变组织中Runx3和c-Myc蛋白表达水平;MTS方法检测TGF-β对骨肉瘤细胞系MG-63细胞增殖能力的影响。结果肉瘤组织中Runx3蛋白的表达水平明显低于骨良性病变组织,而c-Myc则明显高于骨良性病变组织(P<0.05),二者呈负相关(R=-0.797,P<0.05)。TGF-β抑制MG-63细胞增殖活性,并呈剂量依赖关系,TGF-β可显著增强MG-63细胞Runx3蛋白表达并抑制c-Myc蛋白表达;高表达Runx3蛋白可减弱TGF-β对MG-63细胞增殖的抑制作用(P<0.05)。结论TGF-β抑制MG-63细胞增殖可能与上调Runx3蛋白表达进一步抑制c-Myc蛋白的表达相关。Objective To study the role of Runx3 and c-Myc proteins in the inhibitory effect of TGF-βon MG-63 cells proliferation.Methods Western blot was used to detect Runx3 and c-Myc protein expression in osteosarcoma and benign bone tissues.MTS method was used to determine the effect of TGF-βon the proliferation of osteosarcoma cell line MG-63.Results The expression level of Runx3 protein was significantly lower in osteosarcoma tissues than in benign bone lesions but higher for c-Myc protein（P〈0.05）,with negative correlation（R=-0.797,P〈0.05）between Runx3 and c-Myc.TGF-βinhibited significantly MG-63 cell proliferation in a dose-dependent manner,but enhanced Runx3 expression and inhibited c-Myc protein expression in MG-63 cells（P〈0.05）.However,the overexpression of Runx3 protein attenuated the inhibition effect of TGF-βon the proliferation of MG-63 cells（P〈0 05）.Conclusion The inhibition effect of TGF-βon MG-63 cells proliferation might be related to upregulation of Runx3 protein and further inhibition of c-Myc expression.

关 键 词：骨肉瘤 RUNT相关转录因子3 C-MYC 转化生长因子Β 

分 类 号：R738.1[医药卫生—肿瘤]