miRNA-542-3p对胃腺癌细胞侵袭转移能力的影响  

The effect of microRNA-542-3p on invasion and migration of human gastric adenocarcinoma

作　　者：王跃[1] 郑志超[1] 谷小虎[1] 赵岩[1] 张峻[1] 郑国良[1] 杨东[1] 孟祥宇[1] 

机构地区：[1]辽宁省肿瘤医院胃外科,辽宁沈阳110042

出　　处：《解剖科学进展》2018年第1期72-75,共4页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金(2015020269)

摘　　要：目的研究microRNA-542-3p(miR-542-3p)在人胃腺癌细胞系及组织中的表达水平及对胃腺癌细胞侵袭转移的影响。方法通过RT-PCR检测miR-542-3p在人正常胃粘膜上皮细胞GES-1及人胃癌细胞系SGC-7901和BGC-823中的表达水平;同时应用RT-PCR检测miR-542-3p在50组患者胃腺癌和癌旁组织中的表达差异。体外实验通过外源转染miR-542-3p的模拟物和抑制剂,分别于24,36,48h检测SGC-7901的转染效率,选取最佳转染时间。应用划痕实验和Transwell评估细胞侵袭转移能力的变化。结果胃腺癌细胞系SGC-7901和BGC-823中miR-542-3p的表达水平低于其在GSE-1中的表达水平(P<0.05);miR-542-3p在癌组织中表达低于癌旁组织(P<0.05)。24 h后,miR-542-3p模拟物组侵袭转移能力显著低于对照组(P<0.05),而miR-542-3p抑制剂组侵袭转移能力显著高于对照组(P<0.05)。结论miR-542-3p在多种人胃癌细胞和胃腺癌组织中呈低表达,miR-542-3p抑制胃腺癌SGC7901细胞的侵袭转移能力。Objective To study the effect of micro RNA-542-3p（mi R-542-3p）on invasion and migration of human gastric adenocarcinoma.Methods RT-PCR was used to detect the relative expression of mi R-542-3p in gastric adenocarcinoma and normal gastric mucosa cells,RT-PCR was performed to detect the expression of mi R-542-3p in 50cases of gastric adenocarcinoma and the corresponding adjacent non-tumor tissues.The expression of mi R-542-3p in SGC-7901 cell transfected with mi R-542-3p mimics or inhibitor and its negative control was determined by RT-PCR.The influence of mi R-542-3p on the migration and invasion of gastric cancer cell line SGC-7901was detected by Transwell assay and wound healing.Results The relative expression level of mi R-542-3p in gastric adenocarcinoma cells was significantly lower than that in normal gastric mucosa cells（P〈0.05）,lower in cancer tissues than in adjacent non-tumor tissues（P〈0.05）.After transfectiion for 24h,the migration and invasion ability was significantly lower in mi R-542-3p mimics group than in control group（P〈0.05）,but higher in mi R-542-3p inhibitor group than in control group（P〈0.05）.Conclusion mi R-542-3p was downexpressed in gastric adenocarcinoma,and might play a role in inhibiting the migration and invasion of gastric adenocarcinoma cells.

关 键 词：人胃腺癌 miR-542-3p 侵袭 转移 

分 类 号：R735.2[医药卫生—肿瘤]