lncRNA H19对人非小细胞肺癌增殖及转移的影响  

Effect of long non-coding RNA H19 on proliferation and migration of non-small cell lung cancer

作　　者：金文静 任涛[1] 卢青纯 朱冬伊 

机构地区：[1]同济大学附属东方医院呼吸内科,上海200120

出　　处：《国际呼吸杂志》2018年第4期247-251,共5页International Journal of Respiration

摘　　要：目的探讨长链非编码RNA（lncRNA）H19对非小细胞肺癌细胞株A549和H1299的增殖及转移的影响。方法用荧光定量聚合酶链反应法测定并对比A549、H1299和正常人支气管上皮细胞HBE中H19的表达量;用lipo3000转染技术将si-H19转染进细胞后,用CCK-8及EdU测定细胞增殖率,用Transwell法测定细胞转移能力。结果和HBE细胞相比,H19在肺癌细胞株A549和H1299中表达量明显上;CCK-8及EdU实验提示H19被抑制后,细胞增殖率较前下降;Transwell结果表明当H19下调时肺癌细胞的转移能力下降。结论H19在人非小细胞肺癌细胞株A549及H1299中呈高表达,并调控了细胞的增殖及转移。Objective To explore the effect of long non-coding RNA （lncRNA） H19 on the proliferation and migration of non-small cell lung cancer cell lines A549 and H1299. Methods Fluorescence quantitative polymerase chain reaction was used to detect the expression of H19 in A549, H1299 and human bronchial epithelial cell line HBE. After si-H19 was transfected into cells by 1ipo3000, CCK-8 and EdU assays were used to detect the cell proliferation rate, transwell assay was used to detect the cell migration rate. Results Compared with HBE cell line, the expression of H19 was increased in A549 and H1299 cells. The cell proliferation and migration rates were decreased when H19 was knockdown. Conclusions H19 level is increased in non-small cell lung cancer cell lines A549 and H1299, and it modulates proliferation and migration of cells.

关 键 词：非小细胞肺癌 长链非编码RNA H19 细胞增殖 肿瘤转移 

分 类 号：R734.2[医药卫生—肿瘤]