非小细胞肺癌患者外周血Treg和Th17水平及意义  

Treg and Th17 Cells in Peripheral Blood of Patients with Nonsmall Cell Lung Cancer and Their Signification

作　　者：林昌海[1] 黄露迷[1] 冀晓辉[1] 王春梅[1] 王东林[1] 李代蓉[1] 

机构地区：[1]重庆市肿瘤研究所

出　　处：《肿瘤学杂志》2018年第1期27-30,共4页Journal of Chinese Oncology

基　　金：重庆市卫计委项目(2010-2-305,2011-2-350);重庆市自然科学基金(#2013jcyj A10125)

摘　　要：[目的]探讨非小细胞肺癌（NSCLC）患者外周血中Treg和Th17细胞的变化及其意义。[方法]收集40例NSCLC患者和20名健康对照的外周血样本,采用流式细胞术检测外周血中Treg和Th17细胞占CD4＋T细胞的比例,酶联免疫吸附实验法检测TGF-β和IL-17水平。[结果]肺癌患者外周血中Treg细胞和Th17细胞比例均显著性高于健康对照组（分别为6.65%±3.70%vs 4.06%±1.23%和1.20%±1.19%vs 0.44%±0.13%,P〈0.05）。外周血Treg和Th17细胞表达与NSCLC患者分期等无相关性（P〉0.05）。肺癌患者外周血TGF-β和IL-17水平明显高于对照组（分别为25.05±3.19 vs 15.96±3.65和4.36±2.79 vs 2.01±1.83,P〈0.05）。[结论]NSCLC患者外周血中Treg和Th17细胞以及相关细胞因子均高表达,Treg细胞以及Th17细胞参与了肺癌的发生和发展。[Objective] To determine the levels of Treg and Th17 cells in peripheral blood of patients with non-small cell lung cancer（NSCLC） and their significance. [Methods] The peripheral blood samples of 40 NSCLC patients and 20 health subjects were collected. The Treg and Th17 cells in peripheral blood were detected by flow cytometry,the serum TGF-β and IL-17 levels were measured by ELISA.The relationship between the expression of Treg and Th17 cells and clinicopathological features of NSCLC patients was analyzed.[Results] The expression of Treg and Th17 cells in peripheral blood of NSCLC patients were significantly higher than that in healthy controls（6.65%±3.70% vs 4.06%±1.23% and 1.20%±1.19% vs 0.44%±0.13%,both P0.05）.However the levels of Treg and Th17 were not correlated with the stage of NSCLC（P0.05）.The serum TGF-βand IL-17 levels in NSCLC patients were significantly higher than those in controls（25.05±3.19 vs15.96±3.65 and 4.36±2.79 vs 2.01±1.83,both P0.05）. [Conclusion] Treg and Th17 cells and their associated cytokines TGF-β and IL-17 are highly expressed in peripheral blood of NSCLC patients.

关 键 词：TREG细胞 TH17细胞 非小细胞肺癌 肿瘤免疫 

分 类 号：R734.2[医药卫生—肿瘤]