紫菜多糖对肠道病毒71型乳鼠模型T细胞亚群及细胞因子的免疫干预作用  

Immune intervention of porphyra polysaccharide to T cell subsets and cell factor in suckling mouse model of enterovirus 71 infection

作　　者：田冰 刘璟 

机构地区：[1]重庆市三峡中心医院,重庆404000 [2]重庆市三峡医药高等专科学校,重庆404000

出　　处：《中国生物制品学杂志》2018年第2期145-149,共5页Chinese Journal of Biologicals

摘　　要：目的探讨紫菜多糖在肠道病毒71型(enterovirus 71,EV71)乳鼠模型中的免疫干预作用。方法取7日龄健康乳鼠40只,对30只经腹腔注射EV71国际标准株(VR-1432)200μL(约300 TCID50)/只,建立EV71感染模型,其中20只设为干预组,于建模24 h后用紫菜多糖灌胃,剂量为40 mg/(kg·d),其余10只为模型组,分别于接种病毒后第3和7天观察乳鼠的临床表现,并经眼球摘取采血,分离血清。同时设对照组(10只,未注射病毒)。流式细胞术检测T细胞亚群,ELISA法检测干扰素α(interferonα,IFNα)、肿瘤坏死因子-α(tumor ne-crosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)及IL-10的表达水平。结果模型乳鼠经紫菜多糖干预后,显效5只,有效13只,无效1只,死亡1只。注射EV71后第7天,与模型组比较,干预组乳鼠的CD4^+及CD8^+T细胞比例明显升高(P<0.01),CD4^+/CD8^+比值明显降低(P<0.05);IL-6及TNF-α水平明显降低(P<0.001),IL-10及IFNα水平明显升高(P<0.001)。结论紫菜多糖在EV71乳鼠模型中,可诱导T细胞活化,促进IFNα等抗炎细胞因子的分泌,调节免疫功能,对EV71感染可起到正面免疫干预作用。Objective To investigate the immune interventidn of porphyra polysaccharide in suckling mouse model of enterovirus 71 （EV71） infection. Methods Forty healthy suckling mice at age of 7 d were selected, of which 30 were injected i.p. with international standard EV71 VR-I432 strain, 200 μL （about 300 TCID50） for each, to establish the model of EV71 infection, while the other 10 were untreated and served as normal control. Twenty of the model mice were served as test group, which were treated with porphyra polysaccharide at a dosage of 40 mg / （kg · d） by gavage 24 h after the establishment of model, while the other 10 were untreated and served as model control. The clinical manifestations of suckling mice in three groups were observed on days 3 and 7 after inoculation of EV71. The serum samples were collected and determined for T cell subsets by flow eytometry, and for the expression levels of interferon α （IFNα）, tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6） and IL-10 by ELISA. Results After immune intervention by porphyra polysa- ccharide, significant efficiency, efficiency, inefficiency and death occurred in 5, 13, 1 and 1 mice respectively. The proportions of CD4~ and CD8~ T cells in test group on day 7 after injection with EV71 increased significantly （P 〈 0. 01 ）, while the CD4^＋/CD8^＋ decreased significantly （P 〈 0. 05）, the IL-6 and TNF-a levels decreased significantly （P 〈 0. 001 ）, and IL-10 and IFNa levels increased significantly （P 〈 0. 001 ）. Conclusion Porphyra polysaccharide may induce T cell activation, promote the secretion of anti-inflammatory cytokines such as IFNα and regulate the immune function in suckling mice infected with EV71, which plays an role of immune intervention in EV71 infection.

关 键 词：手足口病 肠道病毒71型 紫菜多糖 免疫干预 

分 类 号：R392.12[医药卫生—免疫学] R392.33[医药卫生—基础医学]