根管充填糊剂纳米氢氧化钙制备方法及其对根管微渗漏的影响  

Manufacturing method and effect on endodontic microleakage of nanometer calcium hydroxide root canal filling material

作　　者：王籽明 孙青 杨柳[1] 范伟[1] 

机构地区：[1]武汉大学口腔医学院牙体牙髓科,湖北武汉430079

出　　处：《海南医学》2018年第4期480-485,共6页Hainan Medical Journal

基　　金：国家大学生创新创业基金(编号:201610486111)

摘　　要：目的探讨根管充填糊剂纳米氢氧化钙的制备方法及其对根管微渗漏的影响。方法改良制备纳米氢氧化钙的装置,以Ca Cl2和Na OH作为沉淀反应物,聚乙二醇为改性剂,超声波为辅助振动,正丁醇共沸蒸馏法干燥分散制得纳米氢氧化钙粉体,将粉体与医用碘仿和甘油调制成适合临床根管治疗要求的根管充填糊剂,对其进行表征和葡萄糖微渗漏检测,观察纳米氢氧化钙的理化特性和生物功能性。结果本实验搭建的制备装置自动化程度高,隔菌防尘,明显缩短制备时间,节省了人力和物力;本方法制备出具有明显氢氧化钙和聚乙二醇特征吸收波峰,粒径为100~200 nm,大小均匀,且分散的符合临床根管治疗充填材料基质要求的纳米氢氧化钙;纳米氢氧化钙根管充填糊剂能够显著防止微渗漏发生,在使用初期几乎没有微渗漏发生,随时间延长,微渗漏值增加并不明显。与临床上常规使用的氢氧化钙根充糊剂Vitapex比较,在相同时间段,其防止葡萄糖微渗漏值显著减小,在第28天,纳米氢氧化钙根充糊剂的葡萄糖微渗漏值为(2.09±0.89)mmol/L,显著低于Vitapex的(5.04±0.98)mmol/L(P<0.01)。结论本实验改良的制备装置科学合理,制备的粉体为纳米级、分散均匀的氢氧化钙,作为根充糊剂具有显著防止微渗漏的特性,其理化特性和生物功能性完全符合根管治疗充填材料基质的纳米氢氧化钙的要求。Objective To investigate the manufacturing method and effect on endodontic microleakage of nanometer calcium hydroxide root canal filling material. Methods The nanometer calcium hydroxide（Ca（OH）2） powers were produced by an improved manufacturing system： Ca Cl2 and Na OH as precipitation reactant, polyethylene glycol（PEG） as modifier, ultrasonic wave for auxiliary vibration, azeotropic distillation with n-butanol for desiccation. The powders were then mixed with medical iodoform and glycerol to prepare nano-Ca（OH）2 root canal filling paste with the same requirements of clinic root canal therapy. The physical and chemical characteristics of nano-Ca（OH）2 were observed by infrared spectroscopy and transmission electron microscopy, and endodontic microleakage status was detected by quantitative tests for glucose. Results The improved method of producing nano-Ca（OH）2 had high automaticity and can keep away bacteria and dust, with decreased manufacture time, work intensity and cost. The nano-Ca（OH）2 produced had obvious Ca（OH）2-and PEG-characterized wave peak, in size of 100~200 nm, and were uniformly dispersed, which was qualified for root canal filling. The nano-Ca（OH）2 as root canal filling material can significantly prevent endodontic microleakage, and it had nearly no microleakage at early stage and had no obvious increase in the microleakage value with time prolongation. Compared with ordinary Ca（OH）2 root canal filling material like Vitapex, the value of endodontic microleakage was markedly decreased with effect of nano-Ca（OH）2 root canal filling material： nano-Ca（OH）2（2.09±0.89） mmol/L vs Vitapex（5.04±0.98） mmol/L（P0.01） on 28 days. Conclusion The upgraded manufacturing method is scientific and reasonable, and can produce nano-level, and uniformly dispersed Ca（OH）2 powder, which can significantly prevent endodontic microleakage and are qualified as root canal filling material.

关 键 词：纳米氢氧化钙 根管充填材料 制备方法 表征 根管微渗漏 

分 类 号：R781.33[医药卫生—口腔医学]