凋亡相关因子XAF-1和XIAP的表达及与膀胱癌复发的相关性研究  

Expression of apoptosis related factors XAF-1 and XIAP and their cor-relation with bladder cancer recurrence

作　　者：张庆德 蔡学辉 潘勇 姜文元 柳勇 吴永进 王健 

机构地区：[1]辽宁省瓦房店市中心医院泌尿外科,辽宁瓦房店116300

出　　处：《中国现代医生》2018年第6期24-27,F0003,共5页China Modern Doctor

基　　金：辽宁省大连市卫生局资助项目(1211120)

摘　　要：目的探讨凋亡抑制蛋白XIAP和XIAP相关因子1(XAF-1)的表达及与膀胱癌复发的相关性。方法以第一次复发的20例膀胱癌患者(复发组)和其对应的20例初发的膀胱癌患者(初发组)为研究对象,对照组为20例复发的膀胱癌患者的正常膀胱组织。采用免疫组化方法(SABC)检测XIAP和XAF-1在初发组、复发组、对照组中的表达水平,分析与膀胱癌进展和复发的关系,同时分析XIAP和XAF-1与肿瘤数目、是否肌层浸润、临床分期及恶性程度分级的相关性。结果在初发组与复发组中XIAP的表达强度与临床分期、恶性程度分级呈正相关(P<0.05);即XIAP在不同的临床分期、恶性程度分级的表达强度不同,分期越高,表达强度越强。在初发组与复发组中XAF-1的表达强度与临床分期、恶性程度分级呈负相关(P<0.05),即XAF-1在不同的临床分期、恶性程度分级的表达强度不同,分期越高,表达强度越低。XIAP、XAF-1的表达强度在肿瘤数目、是否肌层浸润均无明显相关性(P>0.05)。对照组、初发组、复发组XIAP指标与XAF-1指标相关(P<0.01),呈负相关,XIAP指标越高,XAF-1指标相对低。结论 XIAP异常高表达和XAF-1异常低表达可能在膀胱癌发病和病情进展中起重要作用,为膀胱癌的治疗和判断预后提供了新思路。Objective To investigate the expression of apoptosis related factors XAF-1 and XIAP and their correlation with bladder cancer recurrence. Methods 20 patients with recurrent bladder cancer in the first time（recurrent group） and 20 patients with primary bladder cancer（primary group） were selected as the study subjects. The control group con- sisted of 20 normal bladder tissues of patients with recurrent bladder cancer. The expression levels of XIAP and XAF- 1 in primary, recurrent and control groups were detected by immunohistoehemistry（SABC）, and their relationship with bladder cancer progression and relapse was analyzed. At the same time, the correlation between XIAP, XAF-1 and the number of tumors, whether myometrial invasion, clinical stage and grade of malignancy was analyzed. Results The ex- pression level of XIAP was positively correlated with clinical stage and malignant grade in the primary group and the recurrent group（P〈O.05）, that was, the expression level of XIAP in different clinical stages and malignant grade was dif- ferent, and the higher the staging, the stronger the expression intensity. The expression intensity of XAF-1 in primary group and recurrent group was negatively correlated with clinical stage and malignant grade（P〈0.05）, that was, the expression level of XAF-1 in different clinical stages and malignant grade was different, and the higher the staging, the weaker the expression intensity. The expression intensity of XIAP and XAF-1 was not significantly correlated with the number of tumors and myometrial invasion （P〉0.05）. The levels of XIAP in control group, primary group and recur- rent group were negatively correlated with XAF-1 （P〈0.01）. The higher the XIAP, the lower the XAF-1. Conclusion Abnormal high expression of XIAP and abnormal low expression of XAF-1 may play an important role in the pathogen- esis and Drozression of bladder cancer and provide a new idea for the treatment and prognosis of bladder cancer.

关 键 词：XIAP XAF-1 膀胱癌 复发 

分 类 号：R737.14[医药卫生—肿瘤]