异丙酚影响基质金属蛋白酶对肺腺癌细胞株增殖侵袭能力的作用  

Effects of propofol on proliferation and invasion of lung adenocarcinoma cell line by matrix metalloproteinases

作　　者：张永强[1] 刘俊[1] 陈胜阳[1] 刘国泽[1] 田建民[1] 岳修勤[1] 

机构地区：[1]新乡医学院第一附属医院麻醉科,河南新乡453100

出　　处：《实用医学杂志》2018年第3期357-361,共5页The Journal of Practical Medicine

基　　金：2016年河南省医学教育研究课题项目(编号:Wjlx2016067)

摘　　要：目的分析异丙酚对肺癌癌细胞增殖侵袭的作用及可能机制。方法肺癌癌细胞体外培养,分为实验对照组、L组、M组、H组。CCK-8细胞增殖实验检测各组癌细胞的光密度值,同时沉默MMP-2基因,观察各组癌细胞增殖活性的变化;Transwell侵袭试验分析各组癌细胞侵袭抑制率的改变及沉默MMP-2后各组差异;Western blot及Q-PCR检测四组细胞MMP-2蛋白及m RNA含量。结果 CCK-8细胞增殖实验显示H组癌细胞活力最低(P<0.05);沉默MMP-2基因后,异丙酚抑制肺癌细胞增殖的作用明显加强(P<0.05);异丙酚处理后的肺癌癌细胞侵袭抑制率较高(P<0.05),且沉默MMP-2基因后其抑制率有所上升;Western blot及Q-PCR实验结果显示:H组细胞MMP-2含量最低(P<0.05)。结论异丙酚可抑制肺癌癌细胞的增殖和侵袭,其作用机制可能与下调MMP-2相关,具有潜在临床治疗价值。Objective To analyze the effect and possible mechanism of propofol on proliferation and invasion of lung cancer cells. Methods Lung cancer cells were cultured and divided into experimental control group, groupL, group M and group H. The optical density of cancer cell was detected by CCK-8. Meanwhile,the difference of the proliferation of cancer cells in each groupwas determined after down-regulation gene. The change of inhibition rate in four groups was analyzed by Transwell test. The expression of MMP-2 and mRNA in different groups were compared by Western blot and Q-PCR. Results the results of CCK-8 test showed the cancer cell viability in H group was lowest and the inhibition ability increasedafter silencing MMP-2 gene（P〈0.05）; the invasion inhibition rate of the lung cancer cells treated with propofol was higher（P〈0.05）, and invasion inhibition rate in each group also rise after silencing MMP-2; Western blot and Q-PCR show that the MMP-2 expression level in group Maud group H were lowest（P〈0.05）. Conclusion Propofol may inhibit the proliferation and invasion of lung cancer cells and its mechanism may be associated with down-regulation MMP-2, which is expected to inhibit the proliferation of lung cancer invasion.

关 键 词：异丙酚 肺腺癌 基质金属蛋白酶2 

分 类 号：R734.2[医药卫生—肿瘤]