大蒜素对大鼠冠状动脉微栓塞后心肌凋亡的影响  

Effect of pretreatment with diallyltrisulfide on cardiomyocyte apoptosis after coronary microembolization in rats

作　　者：梁家宝[1] 李浪[1] 贺文慨 

机构地区：[1]广西医科大学第一附属医院心脏病研究所,南宁530021

出　　处：《实用医学杂志》2018年第3期371-375,共5页The Journal of Practical Medicine

基　　金：国家自然基金项目(编号:81600283)

摘　　要：目的探讨大蒜素(DATS)对大鼠冠状动脉微栓塞(CME)后对心肌凋亡的影响及意义。方法经左心室注射42μm微栓塞球建立CME模型后存活的SD大鼠20只,随机分为单纯微栓塞组(CME组)与大蒜素预处理组(DATS组),每组10只。DATS组术前连续7d给予40 mg/(kg·d)腹腔注射。另选经左心室注射等量生理盐水后存活SD大鼠10只为假手术组(Sham组)。术后6h行心脏超声检查,TUNEL染色检测心肌凋亡,应用实时定量PCR及免疫印迹法检测各组大鼠心肌的蛋白激酶B(Akt)、含半胱氨酸的天冬氨酸蛋白水解酶3(Caspase-3)表达,并采集血清检测大鼠心肌酶c Tn I的水平。结果与Sham组比较,CME组和DATS组心功能明显下降(P<0.05),Caspase-3 m RNA及蛋白表达水平明显升高(P<0.05),Akt m RNA及蛋白表达水平明显降低(P<0.05),c Tn I水平明显升高(P<0.05)。与CME组比较,DATS组心功能明显改善(P<0.05),Caspase-3 m RNA及蛋白表达水平明显降低(P<0.05),Akt m RNA及蛋白表达水平明显升高(P<0.05),c Tn I水平明显降低(P<0.05)。结论大蒜素预处理可明显减少CME后心肌细胞凋亡并改善心功能,其机制可能是通过激活Akt从而阻断心肌细胞中Caspase-3介导的死亡受体凋亡途径的激活。Objective To investigate the effect of diallyltrisulfidc on rats with myocardial injury after eoronary mieroembolization （CME）. Methods 20 survival of SD rats were randomly divided into CME group （CME group） and diallyhrisulfide pretreatment group （DATSgroup）, and these rats were injected with microspheres （42 μm in diameter） into the left ventricles to induce the model of CME, 10 rats for each group. DATS group was received diallyhrisulfide （DATS） 40 mg/（kg· d） for 7 days before operation. Another 10 survival of SD rats was selected as sham operation group （Sham group）, and these rats were injected with the same dose of normal saline by left ventricles. Cardiac function was assessed by echocardiography and the expression of Akt and caspase-3 of myocardial tissue of rats in each group were detected by TUMEL staining, RT-PCR and Western Blot, while testing the level of cTnI after operation of 6 h. Results Compared with Sham group, the cardiac function of CME group and DATS group was significantly decreased （P〈0.05）, the expression of caspase3 mRNA and protein was significantly increased （P〈0.05）, the expression on Akt mRNA and protein was significantly decreased （P〈0.05） （P〈0.05）. Compared with CME group, the cardiac function of DATS group was significantly improved （P〈0.05）, the expression of caspase3 mRNA and protein was significantly decreased （P〈0.05）, the expression of Akt mRNA and protein was significantly increased （P〈0.05）, cTnI level was significantly decreased （P〈0.05）. Conclusion Diallyltrisulfide pretreatment can significantly reduce the apoptosis of cardiomyocytes after CME and improve cardiac function. The mechanism may be through the inhibition of Akt to activate cardiomyocyte caspase3-mediated death receptor activation pathway.

关 键 词：大蒜素 冠状动脉微栓塞 心肌损伤 凋亡 

分 类 号：R54[医药卫生—心血管疾病]