应用酵母双杂交系统筛选LKB1相互作用蛋白  

Screening proteins interacting with human LKB1 by yeast two hybird system

作　　者：徐卿 李洪涛[2] 林峰[2] 姚阳[2] 

机构地区：[1]苏州大学附属第三医院肿瘤科,苏州215006 [2]上海交通大学附属第六人民医院肿瘤内科,上海200233

出　　处：《安徽医科大学学报》2018年第2期232-235,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金青年科学基金(编号:81102038)

摘　　要：目的应用酵母双杂交技术研究与抑癌基因LKB1发生相互作用的蛋白。方法应用酵母双杂交系统,以人LKB1为诱饵,筛选人类全基因组开放阅读框酵母双杂交文库,寻找能与之相互作用的蛋白,并且通过免疫共沉淀和GST-Pull down实验证实其之间的相互作用。结果经过酵母双杂交共获得17个克隆,经过验证分析,最终确定1个无重复阳性克隆。结论获得的1个基因编码的蛋白可能揭示LKB1新的作用机制。Objective To study interaction proteins of LKB1 by yeast two-hybrid system. Methods Using LKB1 as bait,human ORFeome Y2H library was screened and the proteins interacting with LKB1 were searched, and confirmed by immunoprecipitation and GST-PuI1 down experiments. Results One true positive clones from yeast clones were obtained after verification. The results of Co-IP and GST-Pull downillustrated that HERC3 interacted with LKB1. Conclusion The encoding proteins we obtained may play important roles in finding the new function of LKB1.

关 键 词：LKB1 HERC3 酵母双杂交 泛素化 

分 类 号：R730.3[医药卫生—肿瘤] Q816[医药卫生—临床医学]