基于大鼠过敏性哮喘模型的哮喘方主体工艺优选  

Optimization of Main Extraction Process of Xiaochuan Prescription Based on Allergic Asthma Model of Rats

作　　者：康小东[1] 常秀娟[1] 胡晗绯[1] 周恩丽[1] 付娟[1] 王星星[1] 李淼[1] 吴云[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室

出　　处：《中国实验方剂学杂志》2018年第4期21-26,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家“重大新药创制”科技重大专项(2013ZX09402203)

摘　　要：目的：通过大鼠过敏性哮喘模型药效试验优选哮喘方的主体工艺,为该复方的后续研究与开发提供参考。方法：以过敏性哮喘模型大鼠支气管肺泡灌洗液（BALF）中细胞总数,血清中白细胞介素-4（IL-4）,IL-5,γ-干扰素（IFN-γ）,免疫球蛋白E（IgE）水平及肺组织形态学变化等药效指标结合药材中指标成分的转移率对哮喘方4条主体工艺进行评价。结果：设计的4条主体工艺均有升高模型大鼠BALF中细胞总数及减轻肺部炎症和肺气肿的作用;与模型组相比,样品4组大鼠血清中IL-4,IFN-γ和IgE水平具有极显著性差异（P〈0.01）,IL-5水平具有显著性差异（P〈0.05）;样品4中盐酸麻黄碱和盐酸伪麻黄碱、升麻素苷和5-O-甲基维斯阿米醇苷、五味子醇甲、马钱苷的转移率分别为58.97%,63.88%,60.26%和62.79%,在设计的4条工艺路线中各指标成分转移率均最高。结论：在药效筛选试验及指标成分转移率评价中均以工艺路线4为最好,建议可将其作为哮喘方的主体工艺路线。Objective： To optimize the main extraction process of Xiaochuan prescription based on allergic asthma model of rats.Method： The total number of cells in bronchoalveolar lavage fluid（BALF）,levels of interleukin-4（IL-4）,IL-5,interferon-γ（IFN-γ）,immunoglobulin E（IgE） in serum,morphological changes of lung tissue and other pharmacodynamics indexes on model rats combined with transfer rates of index components in medicinal materials were used as evaluating indicators for four extraction processes of Xiaochuan prescription.Result： The four main processes all can increase the total number of cells in BALF and reduce the role of lung inflammation and emphysema in the model rats; compared with the model group,the levels of IL-4,IFN-γ and IgE in the fourth process were significantly different（P〈0.01）,and the level of IL-5 was signigicantly different（P〈0.05）; the total transfer rate of ephedrine hydrochloride and pseudoephedrine hydrochloride was 58.97%,the total transfer rate of prim-O-glucosylcimifugin and 5-O-methylvisammioside was63.88%,the transfer rate of schisandrin was 60.26% and transfer rate of loganin was 62.79% in the fourth process; the fourth process had the highest transfer rates of all index components.Conclusion： The fourth process group is the best by evaluating in the efficacy screening test and transfer rates of index components,and it can be used as the main extraction process of Xiaochuan prescription.

关 键 词：哮喘方 药效试验 提取工艺 指标成分 哮喘 支气管肺泡灌洗液 白细胞介素 

分 类 号：R22[医药卫生—中医基础理论] R283.6[医药卫生—中医学]