龙血竭对实验树鼩体外心肌缺血再灌注模型的影响  

Effect of Dracaenae Cochinchinensis Resina on Experimental Tree Shrews’ Myocardial Ischemia Reperfusion Model in Vitro

作　　者：杨天睿[1,2] 张荣平 穆宁晖[1] 

机构地区：[1]云南省第一人民医院,昆明650032 [2]昆明医科大学药学院,昆明650500 [3]昆明医科大学生物医学工程中心,昆明650500

出　　处：《中国实验方剂学杂志》2018年第4期137-142,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：云南省内设研究机构云南省老年病防治研究中心2016年立项课题项目(2016NS204);云南省教育厅科学研究基金项目(2016ZDX033);云南省卫计委医学后备人才培养计划项目(H-201615);云南省应用基础研究(昆医联合专项)项目(2017FE468-112)

摘　　要：目的：基于树鼩体外心肌缺血再灌注模型,探讨龙血竭对心肌缺血再灌注损伤的作用。方法：实验树鼩随机分为空白组,模型组,溶媒组和龙血竭低、中、高质量浓度组（0.25,0.5,1.0 g·L^-1）,采用Langendorff离体心脏灌流系统建立实验树鼩心肌缺血后再灌注模型,灌注结束后立即取下心脏,-20℃冷冻。酶标法测定丙氨酸氨基转移酶（ALT）,天门冬氨酸氨基转移酶（AST）和乳酸脱氢酶（LDH）,免疫抑制法测定肌酸激酶MB（CK-MB）,黄嘌呤氧化酶法测定超氧物歧化酶（SOD）,硫代巴比妥酸染色法测定丙二醛（MDA）,2,3,5-氯化三苯基四唑染色法（2,3,5-triphenyltrazoliumchloride,TTC）测定切片梗死面积,末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法[terminal dexynucleotidyl transferase（TdT）-mediated dUTP nick end labeling,TUNEL]检测凋亡率。结果：龙血竭药物再灌注后,灌注液及组织中AST,ALT,CK-MB,LDH等指标显著下降,SOD活性显著增加,心肌梗死面积和TUNEL凋亡率显著下降（P〈0.05）。结论：用龙血竭进行再灌注,能够抑制过多氧自由基的产生,减少心肌梗死面积和细胞凋亡,具有抗心肌缺血再灌注损伤作用。Objective： To study the effect of Dracaenae Cochinchinensis Resina on experimental tree shrews＇ myocardial ischemia reperfusion model in vitro.Method： The experimental tree shrews＇ myocardial ischemia reperfusion model was established by the Langendorff isolated heart perfusion system.This study contained6 groups,namely blank group,model group,solvent control group and low-dose,medium-dose,high-dose Dracaenae Cochinchinensis Resina（0.25,0.5,1.0 g·L^-1） groups.The hearts were collected and stored at -20 ℃ after reperfusion.Alanine aminotransferase（ALT）,aspartate transaminase（AST） and lactic dehydrogenase（LDH） were measured by enzyme-labeled immunosorbent assay.Creatine Kinase MB（CK-MB）was measured by immunosuppression; Superoxide dismutase（SOD） was measured by xanthinoxidase method;malondialdehyde was measured by thiobarbital staining method; infarct size was measured by 2,3,5-triphenyltrazoliumchloride（TTC） method,and apoptotic rate was measured by terminal dexynucleotidyl transferase（Td T）-mediated dUTP nick end labeling（TUNEL） method.Result： After reperfusion with Dracaenae Cochinchinensis Resina,CK-MB,LDH,ALT,AST in perfusion fluid and tissues significantly reduced,activity of SOD increased,the myocardial infarction area and TUNEL apoptosis rate reduced.Conclusion： Reperfusion with Dracaenae Cochinchinensis Resina had effect against to myocardial ischemia-reperfusion injury by inhibiting the production of excess oxygen free radicals,meanwhile decreasing myocardial infarction area and apoptosis rate.

关 键 词：龙血竭 心肌缺血再灌注 药物效果 实验树鼩 离体模型 

分 类 号：R22[医药卫生—中医基础理论] R24[医药卫生—中医学]