VCA-IgA联合eIF4E检测对鼻咽癌早期诊断的意义  

Early Diagnostic Value of VCA-IgA Combined with eIF4E in the Detection of Nasopharyngeal Carcinoma

作　　者：桑畅 

机构地区：[1]四川省成都市双流区第一人民医院,610200

出　　处：《实用癌症杂志》2018年第3期362-365,共4页The Practical Journal of Cancer

摘　　要：目的探讨EB病毒衣壳抗原IgA(EBV VCA-IgA)联合真核细胞翻译起始因子4E(eukaryotic initiation factor 4E,eIF4E)在鼻咽癌(nasopharyngeal carcinoma,NPC)早期诊断中的应用价值。方法选择NPC患者60例(NPC组),同期收治的鼻腔乳头状瘤、纤维瘤、血管瘤及鼻息肉等良性肿瘤患者45例(对照组)。收集患者年龄、性别、病理分期、淋巴结转移等临床资料,抽取患者清晨空腹外周静脉血,离心分离血清。采用ELISA法检测血清VCA-IgV水平,qPCR法(一步法)检测患者组织中eIF4E mRNA表达水平。结果生化结果显示,NPC组患者血清VCA-IgV阳性率为78.3%(47/60)明显高于对照组为46.7%(21/45);血清VCA-IgA水平与患者临床分期、病理类型和淋巴结转移间无明显关联(P>0.05)。qPCR结果显示,各病理类型患者癌组织中eIF4E mRNA的表达水平间均有差异,晚期鼻咽癌患者以及发生癌细胞分化和淋巴结转移患者组织中eIF4E mRNA表达均明显升高(P<0.01),但淋巴结近端与远端转移患者间无明显差异(P>0.05)。相关性分析结果发现,良性对照组患者2种检测指标间无明显相关性(P>0.05),而鼻咽癌组以上指标间表现出明显的正相关(P<0.01)。结论 VCA-IgV作为NPC的特异性标志物在良性患者中存在一定的假阳性,但结合组织eIF4E表达差异,可进一步提高NPC的诊出率。Objective To investigate the application value of EB virus capsid antigen IgA （EBV VCA-IgA） combined with eukaryotic initiation factor 4E （eIF4E） in the detection of nasopharyngeal carcinoma （NPC）. Methods 60 cases of NPC patients （ NPC group） were selected. 45 cases of benign tumor, such as nasal papilloma, fibroma, hemangioma and nasal polyp were the control group. Age, gender, pathological stage, lymph node metastasis and other clinical data were collected. Extraction of the patient＇S early morning empty peripheral venous blood and centrifuge sera were analyzed. The serum level of VCA-IgV was detected by ELISA, and the expression of eIE4E mRNA in the tissue was detected by qPCR （ 1 step method）. Results Biochemical results showed that the positive rate of serum VCA-IgV in group NPC was 78.3% （47/60） , which was significantly higher than that of control group 46.7% （21/45）. There was no significant correlation between serum VCA-IgA level and clinical stage, pathological type and lymph node metastasis （ P 〉 0.05）. The results of qPCR showed that the expression level of the differences were cancer types of patients with various pathological eIF4E mRNA, eIF4E mRNA expression was significantly increased in patients with advanced nasopharyngeal carcinoma and cancer cell differentiation and lymph node metastasis tissues （ P 〈 0.01 ） , but the lymph node of proximal and distal metastasis of patients with no significant difference （ P 〉 0.05 ）. Correlation analysis showed that there was no significant correlation between the 2 indicators in the benign control group （ P 〉 0.05 ）, but there was a significant positive correlation between the above indicators in nasopharyngeal carcinoma group （P 〈 0.01 ）. Conclusion There may be some false positives of VCA-IgV which is a specific marker of NPC in benign patients, but it can further improve the diagnostic rate of NPC combined with the differences of eIF4E expression in patients＇tissues.

关 键 词：EB病毒 VCA-IgV EIF4E 鼻咽癌 早期诊断 

分 类 号：R739.62[医药卫生—肿瘤]