大豆低丰度蛋白提取物对小鼠免疫功能及抗氧化能力的影响  

Effects of low-abundant soybean protein extracts on the immune function and antioxidant capacity of mice

作　　者：刘明美[1,2] 齐斌[3] 占今舜[1] 陈银岳 赵国琦[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏联合职业技术学院淮安生物工程分院,江苏淮安223200 [3]常熟理工学院生物与食品工程学院,江苏苏州215500

出　　处：《中国农业大学学报》2018年第2期57-63,共7页Journal of China Agricultural University

基　　金：国家"863"计划项目(2013AA102203);国家自然科学基金项目(31572430)

摘　　要：为研究大豆低丰度蛋白提取物对小鼠免疫功能及抗氧化能力的影响,选用21日龄断奶ICR雄性仔鼠180只,随机分为5组,每组36只,试验期28d,每天灌胃大豆低丰度蛋白提取物200μL(以提取物中蛋白含量计,分别为0、0.5、1.0、2.0、4.0mg)。结果表明:1)试验第7天时,处理组小鼠的白细胞、淋巴细胞和中性粒细胞数目均高于对照,且D_(4.0mg)组极显著高于对照(P<0.01)。2)试验第7和28天时,D_(4.0mg)组小鼠的脾脏指数均高于其他试验组;第7天的处理组小鼠脾脏IL-2、TNF-α基因的相对表达量均高于对照。3)第7天的D_(4.0mg)组小鼠血清T-AOC活力低于其他试验组,第28天的处理组均高于对照;第28天的D_(4.0mg)组小鼠肝脏SOD和GPX基因相对表达量均低于其他试验组。研究发现灌胃大豆低丰度蛋白提取物导致试验初期的小鼠发生了过敏反应,尤以D_(4.0mg)组小鼠最明显;试验后期,小鼠的过敏反应得到缓解甚至消失,抗氧化能力得到一定提高。The aim of this study was to investigate the effects of low-abundant soybean protein（LAP）extracts on immune function and antioxidant capacity in mice.A total of 180 weaned mice aged 21 dwere chosen and randomly divided into 5 groups with 36 mice in each group.The trial took 28 days,200μL of LAP extracts with different protein concentration（0,0.5,1.0,2.0 and 4.0 mg protein respectively）were given by gavage administration.The results showed：1）At 7 d,the number of leukocyte,lymphocyte and neutrophils of blood in all treatment groups were higher than in the control.Those in group D（4.0mg） were significantly higher than in the control（P〈0.01）;2）The spleen index in group D（4.0mg） was higher than other experimental groups at 7 dand 28 d;and the relative transcript levels of IL-2 and TNF-αgene in spleen in all treatment groups were higher than those in the control at 7 d;3）The T-AOC of serum in group D（4.0mg） was lower than in other experimental groups at 7 d.The T-AOC of serum in all treatment groups was higher than in the control and the relative transcript levels of SODand GPXgenes in mice liver of group D（4.0mg） were lower than those in other experimental groups at 28 d.In conclusion,the gavage administration of LAP extracts to mice resulted in the allergic reaction of mice at initial test stage and the group D（4.0mg） was most significant,and the allergic reaction was relieved or disappeared at late test stage and the antioxidant ability of mice was increased.

关 键 词：大豆低丰度蛋白 小鼠 免疫功能 抗氧化能力 

分 类 号：S816.4[农业科学—饲料科学]