赤芝担孢子交配型因子鉴定及多样性分析  

Determination and Diversity Analysis of Mating Types of Ganoderma lucidum Basidispores

作　　者：柯斌榕[1] 卢政辉[1] 吴小平[2] 郭丽羡[2] 兰清秀[1] 

机构地区：[1]福建省农业科学院食用菌研究所特色食用菌繁育与栽培国家地方联合工程研究中心,福建福州350014 [2]福建农林大学菌物研究中心,福建福州350002

出　　处：《热带作物学报》2018年第1期145-150,共6页Chinese Journal of Tropical Crops

基　　金：福建省食用菌产业重大农技推广服务试点项目(灵芝;瓶栽银耳创新服务团队)(No.KNJ-153013);食用菌产业农技推广服务体系建设(No.KNJ-153012)

摘　　要：以7个栽培赤芝品种为实验材料,以其原生质体单核化单核菌株为标准测试菌株进行交配型因子多样性分析,并采用三轮杂交法、OWE-SOJ技术及核迁移技术对其中赤芝JC和AL菌株的担孢子单核体交配型因子进行准确测定。结果表明,供试的7个栽培赤芝品种中至少存在8种A因子和8种B因子。通过卡方检验发现赤芝AL、韩芝1号、松杉灵芝及Gl.0032+2菌株的单核体分离符合预期,而血芝和无孢灵芝原生质体单核化菌株,以及赤芝JC担孢子的单核菌株存在偏分离现象。采用OWE-SOJ技术与核迁移试验相互印证,可以确定赤芝的4种担孢子单核体的交配型,赤芝JC为:T1(AXBx)、T2(AyBy)、T3(AyBx)和T4(AXBy),赤芝AL为:T1(AXBx)、T2(AyBy)、T3(AXBY)和T4(AyBx)。The diversity of mating types was analyzed by the protoplast monokaryons of 7 strains of Ganderma lucidum. The mating types of the basidispores of G. lucidum JC and AL were determined by the method of three-cycle mating system, OWE-SOJ technique and nuclear migration test. The results showed that at least 8 A and 8 B factors were found in the 7 test strains. It was found that the monokaryon segregation of AL, Han-zhi No.1, Song-shan and Gl. 0032 ＋2 was as expected by χ2 test, while the different mating types of protoplasted monokaryon of Xue-zhi and Wu-bao, the basidispores of JC did not display the expected segregation ratio by χ2 test. Four mating types of the basidispores of G. lucidum were determined by OWE-SOJ technique and the nuclear migration test, which verified each other. The monokaryons of JC： T1（AXBx）, T2（AyBy）, T3（AyBx） and T4（AXBy）. The monokaryons of AL： T1（AXBx）, T2（AyBy）, T3（AXBY） and T4（AyBx）.

关 键 词：赤芝 交配型 四级性 亲和 

分 类 号：S646[农业科学—蔬菜学]