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出 处:《湖北农业科学》2018年第3期75-77,共3页Hubei Agricultural Sciences
基 金:中央高校科研基金项目(CZP17068)
摘 要:采集新疆地区胡杨(Populus euphratica)树皮,利用碱提法提取其木聚糖。采用水煮处理脱去大部分水溶性物质后,在胡杨树皮中加入10%氢氧化钠溶液(m∶V=1∶5)在70℃下碱提1 h,8层纱布过滤后的碱提滤液加入0.15倍体积的30%双氧水,70℃脱色1 h,最后调pH至中性,加入2倍体积乙醇,醇沉获得木聚糖。结果表明,得到木聚糖的干重收率为4.35%,自提胡杨树皮木聚糖能够作为木聚糖酶试验中的底物使用,具有一定的替代性,且其来源为新疆胡杨树皮,对当地资源开发利用有一定实际意义。The bark of Populus euphratica in Xinjiang was collected,and then extracted xylan by alkali extraction method.The sediment of the bark of Populus euphratica was cleaned up and most of the water-soluble substance was washed away with boiled water. After adding the 10% sodium hydroxide solution(m∶V=1∶5) at 70 ℃ for 1 h,the alkali extract which was filtered by eight-layer gauze was added with 0.15 fold volume of 30% of hydrogen peroxide at 70 ℃ for 1 h,for decolorization. The final pH was adjusted to neutral,with ethanol(V∶V=1∶2) to obtain xylan. The dry-based yield was 4.35%. The results showed that the xylan from Populus euphratica could be used as a substrate in xylanase experiment.
关 键 词:胡杨(Populus euphratica) 资源利用 新疆 木聚糖
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