机构地区:[1]新乡市中心医院妇瘤一科,河南新乡453000 [2]河南省荣康医院妇产科,河南新乡453000 [3]中山大学第一附属医院妇产科,广东广州510000
出 处:《新乡医学院学报》2018年第3期177-181,共5页Journal of Xinxiang Medical University
基 金:广东省自然科学基金资助项目(编号:2014A030310149)
摘 要:目的探讨microRNA-15b(miR-15b)在子宫内膜癌组织中的表达及其对子宫内膜癌细胞增殖的影响。方法选取2013年2月至2017年2月新乡市中心医院收治的87例子宫内膜癌患者手术切除的子宫内膜癌组织,另选取45例因子宫肌瘤行子宫全切术患者的正常子宫内膜组织作为对照,采用实时荧光定量聚合酶链反应(PCR)检测子宫内膜癌组织和正常子宫内膜组织中miR-15b的表达,并分析miR-15b表达与患者临床病理特征的关系。培养人子宫内膜癌HEC-1A细胞,随机分为miR-15b模拟物组、对照序列组和空白对照组,采用实时荧光定量PCR检测各组细胞中miR-15b表达,四甲基偶氮唑盐(MTT)法检测各组细胞增殖能力,流式细胞术检测各组细胞凋亡率和细胞周期变化。结果子宫内膜癌组织和正常子宫内膜组织中miR-15b相对表达量分别为1.32±0.13、2.49±0.16,子宫内膜癌组织中miR-15b相对表达量低于正常子宫内膜组织(t=46.184,P<0.05);子宫内膜癌组织中miR-15b相对表达量与国际妇产科联合会分期、肌层浸润深度和淋巴结转移有关(P<0.05),与年龄、组织学类型、分化程度、是否累及淋巴管和血管无关(P>0.05)。与空白对照组和对照序列组比较,miR-15b模拟物组人子宫内膜癌细胞中miR-15b相对表达量显著增加(P<0.05),培养24、48、72、96 h时细胞增殖能力均降低(P<0.05),细胞凋亡率升高(P<0.05),G_0+G_1期细胞比例显著增加(P<0.05),而G_2+M期细胞比例减少(P<0.05)。空白对照组与对照序列组人子宫内膜癌细胞中miR-156相对表达量、培养12、24、48、72、96 h时细胞增殖能力、细胞凋亡率及G_0+G期、S期和G_2+M期细胞比例比较差异均无统计学意义(P>0.05)。结论 miR-15b在子宫内膜癌组织中呈低表达,上调子宫内膜癌细胞中miR-15b基因表达可抑制细胞增殖,其机制可能与阻滞细胞周期及促进细胞凋亡有关。Objective To investigate the expression of microRNA-15 b( miR-15b) in endometrial carcinoma and its effect on proliferation of endometrial carcinoma cells. Methods Eighty-seven cases of endometrial cancer tissues were selected as the research objects from patients with endometrial cancer undergoing endometrial cancer staging surgery in Xinxiang Central Hospital from February 2013 to February 2017. Another 45 cases of normal endometrial tissues from patients with uterine fibroids undergoing line hysterectomy were collected as the control. The expressions of miR-15b in endometrial carcinoma tissues and normal endometrial tissues were detected by real-time quantitative polymerase chain reaction( PCR) and relationship between the miR-15b expression and clinicopathological feature was analyzed. The HEC^(-1) A cells were cultured and were randomly divided into miR-15b mimics group,control sequence group and blank control group. The expression of miR-15b in HEC-1A cells was detected by real-time quantitative PCR. Tetramethylazolazole blue( MTT) assay was used to detect the cell proliferation. Flow cytometry was used to detect the apoptotic rate and cell cycle. Results The relative expression levels of miR-15b in endometrial carcinoma tissues and normal endometrial tissues were 1. 32 ± 0. 13 and 2. 49 ± 0. 16,respectively. The relative expression level of miR-15b in endometrial carcinoma tissues was lower than that in the normal endometrial tissues( t = 46. 184,P < 0. 05). The relative expression level of miR-15b in endometrial carcinoma was correlated with International federation of gynecology and obstetrics staging,myometrial invasion and lymph node metastasis( P < 0. 05),but it was not correlated with age,histological type,differentiated degree,in filtrating lymph vessel and blood vessel or not. Compared with the blank control group and the control sequence group,the relative expression level of miR-15b in miR-15b mimics group was significantly increased( P < 0. 05),the cell proliferation abilities at cultured 24,4
关 键 词:子宫内膜癌 microRNA-15b 细胞增殖 细胞凋亡
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