回药捆都而(乳香)挥发油对兔视网膜色素上皮细胞形态学的影响  

Experimental study on the effect of Hui prescriptions Kunduer(frankincense) volatile oil on the morphology of rabbit retinal pigment epithelial cells

作　　者：杨晓旭[1] 赵芳芳 黄洁 俞洋[2] 

机构地区：[1]宁夏医科大学中医学院,宁夏银川750004 [2]宁夏医科大学回医药现代化教育部重点实验室、宁夏医科大学中医学院中医五官科教研室,宁夏银川750004

出　　处：《河北中医》2018年第2期256-259,264,共5页Hebei Journal of Traditional Chinese Medicine

基　　金：2017年度宁夏自然科学基金项目(编号:NZ17077);宁夏医科大学2017年度优势学科群建设科研项目(编号:XY201727)

摘　　要：目的观察回药捆都而(乳香)对兔视网膜色素上皮细胞(RPE)的形态学影响。方法采用正常培养基及分别含有5-氟尿嘧啶(5-FU)和捆都而挥发油培养基培养兔RPE后,经固定、脱水、包埋、双铅染色及超薄切片,通过透射电镜(TEM)在不同倍镜下观察正常组、5-FU组及捆都而挥发油组兔RPE形态。结果正常组兔RPE形态规则,细胞膜及核膜完整,边有微绒毛,核染色质分布均匀,细胞器丰富;5-FU组兔RPE体积缩小,形态不规则,核染色体凝聚成团、边聚,胞浆中可见大量空泡,且胞浆中核糖体及粗面内质网明显减少,溶酶体增多,线粒体断裂溶解,空泡形成;与正常组及5-FU组相比,捆都而挥发油组兔RPE呈现出多样化改变,表现为核染色体、细胞器及胞浆基质等凋亡化改变,以及有自噬小体出现的细胞自噬化改变。结论捆都而挥发油可能是通过诱导兔RPE凋亡、坏死与自噬,从而发挥抑制兔RPE增殖的作用。Objective To observe the effects of Hui prescriptions Kunduer(frankincense) volatile oil on the morphology of rabbit retinal pigment epithelial cells(RPE).Methods Cultured rabbit RPEs were cultured in normal medium and containing 5-fluorouracil(5-FU) and Kunduer,respectively.After fixation,dehydration,embedding,double lead staining,and ultrathin sectioning,transmission electron microscopy(TEM) was used to observe the effect of Kunduer volatile oil on morphology of rabbit RPE compared with normal rabbit RPE cells and 5-FU-treated rabbit RPE.Results In normal rabbits,RPE morphology is regular,with complete cell membrane and nuclear membrane,microvilli on the side,uniform nuclear chromatin distribution,and rich organelles.In the 5-FU group,the RPE of rabbits has a reduced size and irregular morphology,and the nuclear chromosomes aggregate into clumps and borders,a large number of vacuoles were observed in the cytoplasm,and the ribosomes and rough endoplasmic reticulum in the cytoplasm were significantly reduced,lysosomes were increased,and mitochondrial breakdown dissolved vacuoles were formed.Compared with the first two groups,the RPE of the Kunduer volatile oil group showed a variety of changes,manifested as changes in apoptotic nuclear chromosome,organelles,and cytoplasmic mechanisms,and autophagic changes in the presence of autophagosomes.Conclusion The Kunduer volatile oil may play a role in inhibiting the proliferation of rabbit RPE by inducing apoptosis,necrosis and autophagy in rabbit RPE.

关 键 词：乳香 油类 挥发 色素上皮 眼 兔 中药药理学 

分 类 号：R774.13[医药卫生—眼科] R285[医药卫生—临床医学]