Potassium Bromate-induced Changes in the Adult Mouse Cerebellum Are Ameliorated by Vanillin  

作　　者：Hajer Ben Saad Dorra Driss Imen Jaballi Hanen Ghozzi Ons Boudawara Michael Droguet Christian Magne Monsef Nasri Khaled Mounir Zeghal Ahmed Hakim Ibtissem Ben Amara 

机构地区：[1]Laboratory of Pharmacology,University of Sfax,Faculty of Medicine,3029 Sfax,Tunisia [2]Laboratory of Enzyme engineering and Microbiology,National Engineering School in Sfax,University of Sfax,B.P.1173,3038 Sfax,Tunisia [3]Centre of Biotechnology of Sfax,P.O.Box 1177,3018 Sfax,Tunisia [4]Anatomopathology Laboratory,University of Sfax,Habib Bourguiba Hospital,3029 Sfax,Tunisia [5]EA 4324 ORPHY,Faculty of Medicine,University of Brest,29238 Brest Cedex,France [6]EA 2219 Geoarchitecture,University of Brest,UFR Sciences&Techniques,29238 Brest Cedex 3,France

出　　处：《Biomedical and Environmental Sciences》2018年第2期115-125,共11页生物医学与环境科学（英文版）

基　　金：supported by the DGRST grant(Direction Générale de la Recherche Scientifique et Technique,Tunisie):[UR/12 ES-13]

摘　　要：Objective The current study aimed to elucidate the effect of vanillin on behavioral changes, oxidative stress, and histopathological changes induced by potassium bromate （KBrO3）, an environmental pollutant, in the cerebellum of adult mice.Methods The animals were divided into four groups： group 1 served as a control, group 2 received KBrO3, group 3 received KBrO3 and vanillin, and group 4 received only vanillin. We then measured behavioral changes, oxidative stress, and molecular and histological changes in the cerebellum.Results We observed significant behavioral changes in KBrO3-exposed mice. When investigating redox homeostasis in the cerebellum, we found that mice treated with KBrO3 had increased lipid peroxidation and protein oxidation in the cerebellum. These effects were accompanied by decreased Na＋-K＋ and Mg2＋ ATPase activity and antioxidant enzyme gene expression when compared to the control group. Additionally, there was a significant increase in cytokine gene expression in KBrO3-treated mice. Microscopy revealed that KBrO3 intoxication resulted in numerous degenerative changes in the cerebellum that were substantially ameliorated by vanillin supplementation. Co-administration of vanillin blocked the biochemical and molecular anomalies induced by KBrO3.Conclusion Our results demonstrate that vanillin is a potential therapeutic agent for oxidative stress associated with neurodegenerative diseases.Objective The current study aimed to elucidate the effect of vanillin on behavioral changes, oxidative stress, and histopathological changes induced by potassium bromate （KBrO3）, an environmental pollutant, in the cerebellum of adult mice.Methods The animals were divided into four groups： group 1 served as a control, group 2 received KBrO3, group 3 received KBrO3 and vanillin, and group 4 received only vanillin. We then measured behavioral changes, oxidative stress, and molecular and histological changes in the cerebellum.Results We observed significant behavioral changes in KBrO3-exposed mice. When investigating redox homeostasis in the cerebellum, we found that mice treated with KBrO3 had increased lipid peroxidation and protein oxidation in the cerebellum. These effects were accompanied by decreased Na＋-K＋ and Mg2＋ ATPase activity and antioxidant enzyme gene expression when compared to the control group. Additionally, there was a significant increase in cytokine gene expression in KBrO3-treated mice. Microscopy revealed that KBrO3 intoxication resulted in numerous degenerative changes in the cerebellum that were substantially ameliorated by vanillin supplementation. Co-administration of vanillin blocked the biochemical and molecular anomalies induced by KBrO3.Conclusion Our results demonstrate that vanillin is a potential therapeutic agent for oxidative stress associated with neurodegenerative diseases.

关 键 词：CEREBELLUM Behavior Potassium bromate VANILLIN ATPASES Genes expression 

分 类 号：R114[医药卫生—卫生毒理学]