甘蓝型油菜抗寒性鉴定方法的建立和种质资源筛选  

Identification method and selection of cold tolerance in rapeseed ( Brassica napus L. )

作　　者：闫蕾 蔡俊松 高立兵 黄斌 马海清 刘清云 戴熙燕 张学昆[1] 程勇[1] 邹锡玲[1] 

机构地区：[1]中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]湖北省油菜办公室,湖北武汉430060 [3]浠水县农业局油料作物推广站,湖北浠水438200 [4]英山县农业技术推广中心,湖北英山438700 [5]江西南昌安义县鼎湖镇农业技术推广综合服务站,江西安义330500

出　　处：《中国油料作物学报》2018年第1期74-83,共10页Chinese Journal of Oil Crop Sciences

基　　金：国家重点研发计划项目(2017YFD0101700);中国农业科学院科技创新工程;湖北省农业科技创新中心

摘　　要：为筛选可利用的抗寒种质资源,建立了甘蓝型油菜抗寒性鉴定方法:将3叶期到4叶期的幼苗经4℃预处理(低温锻炼)24h,然后分别做3种冷冻处理(-2℃1h、-2℃1h+-4℃1h、-2℃1h+-4℃1h+-6℃1h),随后统计存活率,计算抗寒指数,评估抗寒性。通过该方法对来自国内外的226份油菜资源进行抗寒性评价,结果表明:3种冷冻处理下,供试材料的存活率差别明显,抗寒指数范围是0~52。从中筛选出6份强抗寒性的种质,抗寒指数大于33;根据不同生态区划分,冬性、半冬性和春性材料的平均抗寒指数分别为8.10、7.70和9.10,春性材料抗寒性最优,也说明鉴定方法具有准确性。From available cold tolerance germplasm,identification method was built on rapeseed（ Brassica napus L.）. Seedlings at 3-to 4-leaf stage were tested after 24 h under 4℃ cold acclimation. Survival rates（ SR）and cold tolerance index（ CTI） were inveatigated after 3 treatments（-2℃ for 1 h,-2℃ for 1 h and -4℃ for 1 h,-2℃ for 1 h and -4℃ for 1 h and then -6℃ for 1 h）. A total of 226 materials from domestic and abroad were evaluated. Results showed that the SR of the 3 treatments were from 0-100%,0-80%,and 0-70% respectively. The CTI ranged from 0 to 52. Six strong cold tolerant germplasm were found with CTI of 33 to 52. The results from different ecological zone demonstrated that winter-,semi-winter-and spring-rapeseeds had CTI of 8. 10,7. 70 and 9. 10 respectively. Spring-rapeseeds were more tolerant to cold than winter and semi-winter rapeseeds.

关 键 词：甘蓝型油菜 幼苗期 抗寒指数 抗寒性 

分 类 号：S332.1[农业科学—作物遗传育种] S565.4[农业科学—农艺学]