辐照与粉碎对玉米秸秆酶解葡聚糖转化率的影响  

Effects of Irradiation and Smash on the Glucan Conversion Rate by Enzymatic Hydrolysis of Corn Stalk

作　　者：郭栋豪 陈亮[2] 武小芬[2] 齐慧[2] 王克勤[2] 

机构地区：[1]湖南大学隆平分院,湖南长沙410125 [2]湖南省农业科学院核农学与航天育种研究所农业生物辐照工程技术研究中心,湖南长沙410125

出　　处：《河南农业科学》2018年第1期139-144,154,共7页Journal of Henan Agricultural Sciences

基　　金：公益性行业(农业)科研专项(201503135-12)

摘　　要：为探明辐照剂量与粉碎粒径对玉米秸秆酶解效果的影响,以玉米秸秆为材料,设置0、400、800 k Gy辐照剂量和不同粒径进行酶解试验,测定了不同处理的粒径分布状况和酶解葡聚糖转化率。结果表明,随着辐照剂量提高,样品中小颗粒所占比重逐渐增加,0、400、800 kGy辐照剂量的玉米秸秆在同一粉碎条件下获得粒径r<0.075 mm颗粒的比重依次为15.03%、53.22%、63.09%;0、400 kGy辐照剂量处理玉米秸秆的酶解葡聚糖转化率随粒径的减小而提高,当粒径由r>0.450 mm减小至r<0.075 mm时,转化率分别由16.01%、31.54%显著提高至30.25%、59.71%,且辐照与粉碎对玉米秸秆酶解葡聚糖转化率具有协同作用;800 kGy处理的不同粒径玉米秸秆酶解葡聚糖转化率差异不明显,且辐照与粉碎无协同作用。0、400 kGy辐照下的玉米秸秆,粉碎粒径越小,酶解转化率越高,在优化预处理和酶解糖化工艺等方面具有一定价值。To clarify the effect of irradiation dose and particle size on the enzymatic hydrolysis of corn stalk,different irradiation doses of 0,400,800 kGy and different particle sizes of corn straw were used for enzymatic hydrolysis test,and the particle size distribution and the conversion rate of enzymatic glucan of different treatments were determined. The results showed that the proportion of small particles in the samples increased gradually with the increase of irradiation dose. Under the same pulverization condition,the percentage of r 0. 075 mm particle in 0,400,800 kGy samples were 15. 03%,53. 22%,63. 09% respectively. The conversion rate of hydrolyzed glucan in 0 kGy and 400 kGy treatments increased with the decrease of particle size. When the particle size was reduced from r 0. 450 mm to r 0. 075 mm,the conversion rate significantly increased from 16. 01% and 31. 54% to 30. 25% and 59. 71% respectively,and there were synergistic effects on the enzymatic hydrolysis of corn stalks between irradiation and smash. There was no significant difference in the glucan conversion rate of different particle size treated with 800 kGy,and irradiation and smash had no synergistic effect. The smaller the particle size of the corn stalk under 0 and 400 kGy irradiation,the higher the enzymatic conversion rate,which showed a goodpotential value in pretreatment optimization and enzymolysis saccharification process.

关 键 词：玉米秸秆 Γ射线辐照 粒径 酶解 葡聚糖转化率 

分 类 号：S124.1[农业科学—农业基础科学]