裂褶菌对玉米秸秆木质纤维素的降解作用  

Degradation of Lignocellulose in Corn Stover by Schizophyllum commune

作　　者：刘云鹏[1,2] 苏瑛杰 孙旸[1] 王刚[1] 陈欢[1] 于潇潇[1] 陈光[1] 

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]吉林建筑大学,长春130118

出　　处：《吉林农业大学学报》2018年第1期63-70,共8页Journal of Jilin Agricultural University

基　　金：吉林省高等学校秸秆综合利用高端科技创新平台[吉高平台字(2014)C-1]

摘　　要：采用固体发酵工艺,通过测定玉米秸秆中木质纤维素的含量及发酵后玉米秸秆结构的变化,探究裂褶菌对玉米秸秆中木质纤维素的降解作用,并用气质联相色谱(GC/MS)对木质纤维素的降解产物进行分析。结果表明:裂褶菌可产生4种与木质纤维素降解相关的酶(纤维素酶、木聚糖酶、木素过氧化物酶和锰过氧化物酶),且在发酵48~72 h 4种酶活力均可达到最高值。扫描电镜、X射线衍射及傅里叶红外光谱结果显示,裂褶菌固体发酵对玉米秸秆表面结构、结晶度及基团均有显著影响。此外,GC/MS结果也证实,裂褶菌降解后的玉米秸秆产物中出现了小分子芳香族化合物、烃类、棕榈酸等产物。以上结果表明,裂褶菌对玉米秸秆木质纤维素具有较强的降解能力。Solid fermentation technology was used to estimate degradation of lignin in corn stover by determining the content of lignocellulose and the changes of structure after degradation in corn stover,and the degradation products of lignocellulose were analyzed by gas chromatography-mass spectrometry（ GC/MS）. The results showed that four enzymes related to the degradation of lignocellulose,cellulose,hemicellulose,lignin peroxidase and manganese peroxidase can be produced by Schizophyllum commune and and they could reach the maximum enzyme activity in the fermentation of 48-72 h. Scanning electron microscopy（ SEM）,X-ray diffraction（ XRD） and Fourier transform infrared spectroscopy（ FTIR） results showed that the surface structure,crystallinity and the group of the corn stover were significantly affected by the solid fermentation of Schizophyllum commune. In addition,GC/MS results also confirmed that aromatic compounds,hydrocarbons and palmitic acid have been found in the corn stover after degradation. All these results showed that Schizophyllum commune had strong ability to degrade lignocellulose of corn stover.

关 键 词：裂褶菌 木质纤维素 玉米秸秆 酶 固体发酵 

分 类 号：S154.3[农业科学—土壤学] TQ920.6[农业科学—农业基础科学]