石家庄市学龄儿童龋病细菌类型的核酸序列分析  

DNA sequence analysis of cariogenic bacteria of school-age children residing in Shijiazhuang

作　　者：李鑫楠 王洁 李涛 刘慧娟 徐明 董福生 

机构地区：[1]河北医科大学口腔医学院.河北省口腔医学重点实验室.口腔病理科,石家庄050017 [2]河北医科大学口腔医学院.河北省口腔医学重点实验室.口腔内科,石家庄050017 [3]河北医科大学口腔医学院.河北省口腔医学重点实验室.口腔颌面外科,石家庄050017

出　　处：《现代口腔医学杂志》2018年第1期1-5,共5页Journal of Modern Stomatology

摘　　要：目的探讨儿童龋病发生与致龋菌基因突变的关系。方法采集60名石家庄市8~12岁学龄儿童的口腔牙菌斑样本,设计特异性变异链球菌的引物,进行PCR扩增和核酸序列分析,同时进行龋病的问卷调查。结果学龄儿童在睡前饮料和甜点及刷牙习惯上,差别无显著性。龋病组与无龋组牙菌斑中变异链球菌检出率分别为70%和33.3%,差别有显著性(P<0.05)。无龋组变异链球菌突变率(32.3%~39.2%,平均36.1%)高于龋病组变异链球菌突变率(12.7%~31.3%,平均21.3%),差别有显著性(P<0.05)。结论变异链球菌突变率达到或超过36.1%水平时,致龋能力明显降低(P<0.05)。Objective To research the relationship between gene mutation of cariogenic bacterial and children cariogenesis. Methods Sixty Shijiazhuang school-age children aged from 8 to 12 years old were performed caries investigations and dental plaque collected at Hospital of Stomatology, Hebei Medical University from July 2015 to February 2016. Specific primers were designed for Streptococcus mutans, extraction of dental caries was performed from dental plaque for PCR amplification. PCR positive cases were carried out DNA sequence analysis. Results There was no significant difference in bedtime drinks and dessert as well as brushing habits of school-age children. There was significance difference in mutation rate of caries-disease group（70%） and caries-free group（33.3%）（P〈0.05）. The Streptococcus mutans mutation rate of caries-free group（32.3%~39.2%, average 36.1%）was higher than that of cariesdisease（12.7%~31.3%, average 21.3%）（P〈0.05）. Conclusion When the mutation rate of Streptococcus mutans arrives and/or exceeds 36.14%, the cariogenic ability of Streptococcus mutans decreased significantly（P〈0.05）.

关 键 词：儿童 龋病 变异链球菌 PCR 核酸序列分析 

分 类 号：R780.2[医药卫生—口腔医学]