HDPSCs在体外促进血管再生的潜能及其分子机理研究  

Study on the potential of HDPSCs in promoting angiogenesis in vitro and its molecular mechanism

作　　者：刘景[1] 袁媛[1] 

机构地区：[1]新疆医科大学第五附属医院口腔科,乌鲁木齐830011

出　　处：《现代口腔医学杂志》2018年第1期11-15,共5页Journal of Modern Stomatology

基　　金：新疆维吾尔自治区自然科学基金项目(2016D01C229)

摘　　要：目的探讨人牙髓干细胞(HDPSCs)在体外促进血管再生的潜能及其机制。方法体外分离培养HDPSCs,同时选取人微血管内皮细胞(HMEC)随机分为阴性对照组,阳性对照组和HDPSCs组,其中阴性对照组采用不含胎牛血清(FBS)的-MEM培养基,阳性对照组加入含100ml/l FBS的-MEM培养基,HDPSCs组加入HDPSCs培养。采用MTS法检测HEMC细胞增殖情况,采用Trans-well小室实验检测HEMC细胞迁移能力,采用Western blot法检测ERK1/2、p-ERK1/2、p38和p-p38蛋白表达,并对比各组检测结果。结果阳性对照组培养24h和48h HEMC细胞OD值分别为(0.511±0.099)和(0.624±0.103),明显高于阴性对照组和HDPSCs组(P<0.05);HDPSCs组培养24h和48h HEMC细胞OD值分别为(0.390±0.098)和(0.455±0.096),明显高于阴性对照组(P<0.05);阳性对照组HEMC细胞迁移数为(115.64±12.06)个,明显多于阴性对照组和HDPSCs组(P<0.05);HDPSCs组HEMC细胞迁移数为(75.60±9.81)个,明显多于阴性对照组(P<0.05);阳性对照组ERK1/2、p-ERK1/2、p38和p-p38蛋白相对表达量分别为(0.947±0.037)、(0.867±0.043)、(0.833±0.069)和(0.792±0.086),明显高于阴性对照组和HDPSCs组(P<0.05);HDPSCs组ERK1/2、pERK1/2、p38和p-p38蛋白相对表达量分别为(0.528±0.046)、(0.422±0.050)、(0.554±0.074)和(0.512±0.077),明显高于阴性对照组(P<0.05)。结论 HDPSCs在体外可促进血管内皮细胞增殖和迁移,可能与其参与ERK1/2和p-p38MAPK信号通路有关。Objective To investigate the potential of human dental pulp stem cells（HDPSCs） promoting angiogenesis in vitro and its mechanism. Methods HDPSCs were isolated and cultured in vitro; and human microvascular endothelial cells（HMEC）were selected and randomly divided into negative control group, positive control group and HDPSCs group; the negative control group was added-MEM culture medium without fetal bovine serum（FBS）, the positive control group was added-MEM culture medium with 100 mL/L FBS, and HDPSCs group was added HDPSCs co-culture. MTS method was used to detect the proliferation of HEMC cells, the migration ability of HEMC cells was detected by scarification method, and the expression of p-ERK1/2 and p-p38 protein was detected by Western blot method. Results The OD values of HEMC cells cultured 24 h and 48 h in the positive control group were respectively（0.511±0.099）and（0.624±0.103）, which was significantly higher than that in the negative control group and HDPSCs group（P〈0.05）; The OD values of HEMC cells cultured 24 h and 48 h in the HDPSCs group were respectively（0.390±0.098） and（0.455 ±0.096）, which was significantly higher than that in the negative control group（P〈0.05）; The migration number of HEMC cells in positive control group was（115.64±12.06）, which was significantly higher than that in negative control group and HDPSCs group（P〈0.05）;The migration number of HEMC cells in HDPSCs group was（75.60±9.81）, which was significantly higher than that in negative control group（P〈0.05）;The relative expression of ERK1/2, p-ERK1/2, p38 and p-p38 protein in the positive control group were respectively（0.947 ±0.037）,（0.867 ±0.043）,（0.833±0.069） and（0.792±0.086）, which were significantly higher than those in the negative control group and HDPSCs group（P〈0.05）; The relative expression of ERK1/2, p-ERK1/2, p38 and p-p38 protein in the HDPSCs group were respectively（0.528 ±0.046）,（0.422 ±0.050）,（0.554 ±0.074）

关 键 词：人牙髓干细胞 人微血管内皮细胞 增殖 迁移 ERK1/2 P-P38MAPK 

分 类 号：R780.2[医药卫生—口腔医学]