基于Micro-CT的家兔腹盆腔器官血管数字化三维模型的构建  

Microvascular imaging of abdominal and pelvic organs in rabbit using Micro-computed tomography

作　　者：魏恒 李海[2] 卫兵[1] 张妤[1] 许有江[1] 许胜红 陈川 王文艳[1] 

机构地区：[1]安徽医科大学第二附属医院妇产科,合肥230000 [2]中国科学院合肥物质科学研究院医学物理与技术中心医学影像研究室

出　　处：《中华解剖与临床杂志》2018年第1期1-6,共6页Chinese Journal of Anatomy and Clinics

基　　金：国家自然科学基金（81100412）;中国博士后科学基金（2016M592038）;安徽省公益性研究联动计划项目（5101ID04041）

摘　　要：目的 通过乳胶-氧化铋对比剂与显微计算机断层扫描（Micro-CT）技术相结合的方法,完成家兔腹盆腔器官内血管系统的三维数字化图像重建.方法 6只4月龄雌性健康家兔作为研究对象.试验动物均进行腹腔麻醉.麻醉成功后纵行切开家兔腹壁,暴露盆腹腔血管,完成腹主动脉插管（用于子宫、膀胱、小肠的对比剂在体灌注）及肾动脉插管（用于肾脏的对比剂在体灌注）.选择乳胶-氧化铋混合溶液（氧化铋颗粒/乳胶溶液=1 g/mL）作为血管灌注对比剂.血管灌注成功后选取家兔的子宫、膀胱、小肠和肾脏作为研究器官.利用SkyScan 1076型Micro-CT对目标器官进行扫描,使用NRecon软件对扫描获得的原始图像进行处理和转化.采用Mimics 17.0软件对目标器官内的血管图像进行三维重建,获得家兔不同器官内的血管树三维图像,分析家兔不同器官内不同血管树的分级和分支特点.结果 目标器官的血管树数字化三维重建模型均构建成功.家兔膀胱血管树的最小可测量血管分级为二级,直径为（0.41±0.08）mm;子宫血管树的最小可测量血管分级为三级,直径为（0.39±0.08）mm;小肠和肾脏血管树的最小可测量血管分级均为四级,直径分别为（0.27±0.04）mm和（0.19±0.03）mm.结论 采用乳胶-氧化铋混合对比剂进行动物血管灌注Micro-CT成像,通过相关软件可成功重建动物器官内血管树的三维图像,可显示家兔器官血管树的走行并测量血管直径,对于小动物腹盆腔器官血管系统尤其是微小型血管网数字化三维模型的建立具有较大优势,为小动物腹盆腔器官疾病模型中血管解剖结构的研究提供了新的思路和方法.Objective To construct the three-dimensional digital image of vascular system in abdominal and pelvic organs of rabbits using latex-bismuth oxide contrast agent and micro-computed tomography （Micro-CT）. Methods A total of 6 female healthy rabbits （4-month old） were chosen as the subjects and anesthetized intraperitoneally, followed by abdominal longitudinal incision and exposure to abdominal and pelvic vessels for the completion of abdominal aortic cannulation, which was used for the contrast agent perfusion into the uterus, bladder, small intestine in vivo and renal artery cannulation for renal perfusion. Latex-bismuth oxide mixture was chosen as the contrast agent （ bismuth oxide particles / latex solution =1 g/mL ） . After successful vascular perfusion, the uteruses, bladders, small intestines and kidneys of the rabbits were collected as the subject organs, which were scanned by Micro-CT （ SkyScan 1076, Bruker Corporation, Billerica, UA）, the software NRecon was used to process and transform the original images from Micro-CT, and Mimics 17. 0 was applied to construct the images of the vessels in the targeted organs for obtaining the three-dimensional images of the vascular trees in different organs of the rabbits as well finally, the classification and branch characteristics of the different vascular trees were analyzed. Results The digital three-dimensional reconstruction models of the vascular trees of the targeted organs were completed successfully. The minimum measurable vascular classification of the bladder vascular tree in rabbits was grade Ⅱ with a diameter of （0. 41 ± 0. 08） mm, grade Ⅲ was for the uterus vessels with a diameter of （0. 39 ± 0. 08） mm, and grade Ⅳ for both the small intestine and kidney vessels with diameters of （0. 27 ± 0. 04） mm and （0. 19 ± 0. 03） mm, respectively. Conclusions Micro-CT imaging based on contrast agent of latex-bismuth oxide mixture can successfully establish the three-dimensional images of the vascular trees in animal organs

关 键 词：血管造影术 X线显微体层摄影术 对比剂 成像 三维 兔 模型 动物 

分 类 号：R-332[医药卫生] R816