猪源嗜麦芽寡养单胞菌的分离鉴定与生物学分析  

Isolation,identification and biological characteristics analysis of Stenotrophomonas maltophila

作　　者：张鹏飞[1,2] 王印[1,2] 杨泽晓[1] 姚学萍[1] 张博[1,2] 

机构地区：[1]四川农业大学动物医学院,成都611130 [2]动物疫病与人类健康四川省重点实验室,成都611130

出　　处：《中国农业大学学报》2018年第4期45-52,共8页Journal of China Agricultural University

基　　金："十二五"国家科技支撑计划项目(2013BAD12B04)

摘　　要：从四川省某规模化养猪场病死猪肺脏分离得到1株嗜麦芽寡养单胞菌,分析其生物学特性,为临床鉴别诊断和治疗提供参考。观察分离株的染色特点、培养特性,并通过生化试验进行鉴定;测定分离株对小鼠的LD_(50)并进行药物敏感试验,以分析分离株的致病性与耐药性;测序分析分离株16S rRNA基因序列,构建系统进化发育树。结果表明:该分离株的16S rRNA基因序列与嗜麦芽寡养单胞菌的同源性为99%;在TSA琼脂培养基上培养18h后可长成灰白色、光滑的露珠样小菌落,在血琼脂上菌落周围呈现α溶血;对小鼠的致病性试验测得LD_(50)为6.62×10~7 cfu/只;药敏试验发现分离株对多粘菌素B较为敏感,对喹诺酮类、四环素类、头孢类、碳青酶烯类和氨基糖苷类药物均表现为耐药。经鉴定,分离株为嗜麦芽寡养单胞菌,致病性较强,对多种药物耐药。该研究为该菌的临床诊断、选药治疗、疾病控制等奠定了基础。The aim of this study was to investigate the biological characteristics and provide references for Stenotrophomonas maltophila identification, diagnosis and treatment. The dyeing characteristics and culture characteristics of the isolate was observed and identified by biochemical tests. To determine LD50 of the isolate in mice, drug sensitive test was conducted to analyze the toxicity and drug resistance. A system evolutionary tree was built based on the result of 16S rRNA gene sequence analysis. The results of the 16S rRNA sequencing of the isolated strain showed 99% homology with S. maltophila. The colonies of the isolated strain on TSA agar appeared to be smooth, circular and translucent and had α-hemolysis on blood agar. The LD50 of isolated strain to mice was 6.62 × 107 cfu per mouse. The results of antibiotic sensitivity experiment showed that it was sensitive to cephalosporin antibiotics but insensitive to quinolone, tetracycline, cephalosporin antibiotics, carbapenems and aminoglycoside antibiotics. In conclusion, this pathogen was identified to be S. maltophila. In addition, it was proved to have strong pathogenicity and powerful drug resistance. This study provided references for the clinical diagnosis, disease prevention and drug treatment of associate streptococcus disease. K

关 键 词：嗜麦芽寡养单胞菌 16S RRNA 分离鉴定 人兽共患病 

分 类 号：S855.1[农业科学—临床兽医学]