选择性激光烧结技术制作的双相磷酸钙骨组织工程支架的工艺和生物学性能  

Preparation of Biphasic Calcium Phosphate Scaffolds for Bone Tissue Engineering by Selective Laser Sintering

作　　者：曾浩 王敏[1,2] 陈冬 施斌[1,2] 白轶 

机构地区：[1]武汉大学口腔医学院.湖北省口腔基础医学重点实验室-省部共建国家重点实验室培育基地.武汉大学口腔生物医学教育部重点实验室暋,湖北武汉430079 [2]武汉大学口腔医院种植科,湖北武汉430079

出　　处：《口腔医学研究》2018年第2期165-168,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号：31400837)

摘　　要：目的:利用选择性激光烧结技术制作多孔的双相磷酸钙的骨组织工程支架,并检测其生物相容性。方法:利用选择性激光烧结机和马弗炉,将混匀的环氧树脂和双相磷酸钙粉末进行2次烧结,去除环氧树脂,得到多孔的双相磷酸钙支架,再利用X射线衍射技术和红外光谱法分析材料的组成,使用扫描电镜观察双相磷酸钙支架的表面形貌。接种到支架上的小鼠胚胎成骨细胞前体细胞(MC3T3-E1)培养1周后,使用共聚焦显微镜观察细胞的形貌;培养2周后,CCK-8试剂检测细胞活力,活死细胞染色观察。结果:经过烧结后,双相磷酸钙支架内的环氧树脂被完全去除,支架的表面可观察到多孔结构,MC3T3-E1细胞在材料表面伸展性好。CCK-8检测结果表明,培养10d后,生长在支架的细胞与生长在孔板的细胞活力无差异性。结论:选择性激光烧结技术制作的双相磷酸钙支架具有多孔结构以及良好的生物相容性,可作为骨组织工程支架的备选材料。Objective：To prepare biphasic calcium phosphate scaffolds for bone tissue engineering by selective laser sintering and to evaluate the biocompatibility of the scaffolds.Methods：After fully mix of biphasic calcium phosphate powders and epoxy resin powders,the green parts of biphasic calcium phosphate scaffolds were fabricated by a selective laser sintering machine.Then the epoxy resin was burned during sintering in muffle furnace to get final scaffolds.X-ray diffraction and Fourier transform infrared spectroscopy were used to examine the composition of scaffolds.The scaffolds surface was observed by scanning electron microscope.The morphology of MC3 T3-E1 cells adhered on scaffolds was observed by confocal laser scanning microscope after culture for 7 days.CCK-8 assay and living-dead staining were performed to evaluate the cell growth.Results：After sintering in muffle furnace,the remained epoxy resin was removed.The porous structure could be found on the surface of scaffolds.MC3 T3-E1 cells could distribute well on the surface of scaffolds.The result of CCK-8 assay showed that there was no significant differences on cell viability between cells cultured in scaffolds and in culture plates at day 10.Conclusion：Selective laser sintered biphasic calcium phosphate not only possesses porous structure but also has good biocompatibility.

关 键 词：选择性激光烧结 双相磷酸钙 MC3T3-E1细胞 骨组织工程支架 

分 类 号：R783.1[医药卫生—口腔医学]