基于SCoT标记的陕西茶树种质资源遗传多样性分析  

Analysis on Genetic Diversity of Tea Germplasm in Shaanxi Based on SCoT Markers

作　　者：王令[1] 李佼[2] 席彦军[2] 吴军舰 郭明星[2] 李秀峰[2] 张羽[1] 

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723000 [2]陕西省汉中市农业科学研究所,陕西汉中723000

出　　处：《西北农业学报》2018年第2期244-252,共9页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省科技统筹创新工程计划项目(2016KTCQ02-06);陕西省农业科技创新转化项目(NYKJ-2015-031)~~

摘　　要：为了明确陕西省本地群体种茶树种质资源的遗传多样性,为茶树育种提供依据。采用正交设计和单因素分析方法,对茶树SCoT-PCR体系进行优化。结果表明:20μL最佳反应体系为Mg2+2.5mmol/L,Taq酶0.75U,引物0.7μmol/L,dNTPs 0.25mmol/L,模板DNA 30ng。利用该体系对22份陕南茶树地方品种材料进行分析,扩增条带多且清晰,22条引物共检测出241个等位位点,其中多态性位点228个,多态性比率为94.6%,位点的PIC值为0.57~0.92。供试材料之间的遗传相似系数为0.58~0.89,平均值达到0.72。基于SCoT标记的聚类与茶树叶片形态有很大的相关性。研究表明茶树SCoT体系结果稳定,重复性好,为后续研究茶树资源遗传多样性提供技术支撑。This study aims to unveil the genetic polymorphisms of tea in Shaanxi province,and provide basic data for tea breeding.The orthogonal design table of L16（45）and single factor experiments were designed to optimize the SCoT-PCR（start codon targeted polymorphism）system in tea.Results demonstrated that the optimal reaction system of 20μL contained 2.5 mmol/L Mg2＋,0.75 U Taq DNA polymerase,0.7μmol/L primers,0.25 mmol/L dNTPs,30 ng DNA template.Twenty-two different tea germplasms in Shaanxi province were investigated by the optimized system,and an abundance of bands with different sizes were obtained clearly.Total of 241 fragments were generated with 22 primers in all these samples,and 94.6% of fragments showed polymorphisms.Genetic polymorphism information content of each SCoT locus was between 0.57 and 0.92.The genetic similarity coefficient was between 0.58 and 0.89,with average 0.72.Additionally,the result of dendrogram based on SCoT bands of 22 tea materials illustrated that tea genetic polymorphisms were significantly correlated with leaf morphology.In summary,the established SCoT-PCR system is stable and repeatable,which could be applied to explore genetic diversity of tea germplasm resources in further study.

关 键 词：茶树 SCoT标记 遗传多样性 

分 类 号：S571.1[农业科学—茶叶生产加工]