pH对青蛤的钙化率和两种酶活性的影响  

Effects of p H on calcification,CA and SOD activities in Cyclina sinensis

作　　者：蔡娟 周凯[1] 盛文权[1] 隋延鸣[1] 来琦芳[1] 陆建学[1] 么宗利[1] 高鹏程 

机构地区：[1]中国水产科学研究院东海水产研究所,盐碱水域渔业工程技术研究中心,上海200090 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《海洋渔业》2018年第1期57-64,共8页Marine Fisheries

基　　金：中国水产科学研究院基本科研业务费专项课题：东海区滩涂贝类固碳潜力及pH对其发育的影响研究(2014A01YY02)

摘　　要：pH是影响水生动物的重要环境因子,pH的改变会引起水生动物一系列的生理应答。青蛤(Cyclina sinensis)是我国重要的养殖贝类,其在养殖过程中面临各种pH挑战。为了解青蛤在不同pH下的生理应答,实验设置了5个pH梯度:7.4、7.8、8.2、8.6、9.0,进行了7 d不同pH暴露。结果发现,经过7 d pH暴露,pH7.8组钙化率仅稍微降低,而pH 7.4、pH 8.6、pH 9.0组钙化率变为负值;pH 7.8、pH 8.2、pH 8.6组中各组织CA酶活性在整个实验期间保持稳定,pH 7.4、pH 9.0两组暴露3 h后CA酶活性开始升高并在24 h达到峰值,然后开始下降至120 h恢复至初始水平后保持稳定;与CA酶类似,pH 7.8、pH 8.2、pH 8.6组中各组织SOD酶活性在整个实验期间保持稳定,pH 7.4、pH 9.0两组从暴露12 h后开始升高并在24 h达到峰值,然后开始下降至48 h恢复至初始水平后保持稳定。结果表明,青蛤可以承受较大的pH波动,推测酸碱环境因素变化可能使青蛤产生生理应激效应,导致SOD和CA短期波动,但最后通过其自身调节可以恢复到初始状态。pH is an important indicator of water environment and aquatic animals show a series of physiological responses to pH changing.The Venus clam（Cyclina sinensis） is an intertidal clam that is naturally distributed along the coastal muddy sands of China,Korea,Japan and Southeast Asia.C.sinensis farms have been very popular in China since 1990 s.At the C.sinensis farm,water pH fluctuate wildly.In order to understand the physiological responses of C.sinensis to various pH changes,the experiment was set up with 5 pH gradients： 7.4,7.8,8.2,8.6,9.0 for 7 days.CAT and SOD activities were detected at 0 h,3 h,12 h,24 h,48 h,72 h,120 h,168 h after pH treatment.The calcification rate was evaluated at the end of the experiment.Data showed that there was no significant effect in pH 7.8 group compared with the control group（P 0.05）,but in the other groups the calcification rate were significantly depressed（P 0.05）;Significant differences in SOD and CAT activities were only found in pH 7.4 and 9.0 groups（P 0.05）.The enzyme activities raised after pH challenge and then recovered to the initial level in both pH 7.4 and 9.0 groups.The results indicated the C.sinensis could tolerate a wide range of pH,but pH fluctuation still brought significant variances during the growth of C.sinensis.

关 键 词：青蛤 PH 钙化率 SOD酶活性 CA酶活性 

分 类 号：S917.4[农业科学—水产科学]