西伯利亚蓼EST-SSR引物开发和国内资源的遗传背景分析  

Development of EST-SSR Markers and Genetic Background Analysis for Polygonum sibiricum in China

作　　者：王晓云 徐旭 程波翔 慕泽泾 杜小浪 钟国跃 

机构地区：[1]江西中医药大学中药资源与民族药研究中心

出　　处：《中国实验方剂学杂志》2018年第5期43-49,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江西民族传统药现代科技与产业发展协同创新中心2014年度协同创新项目(JXXT201401007)

摘　　要：目的：评价国内西伯利亚蓼资源的遗传分化程度,为种群分化、物种进化、资源保护和药材品质保障提供科学依据。方法：从国内4个省份13个采样点采集56份西伯利亚蓼,开发并筛选多态性EST-SSR标记,结合相关序列扩增多态性（SRAP）分子标记扩增结果,通过计算遗传距离和聚类分析,评价不同生态分布区域内西伯利亚蓼的遗传分化程度。结果：开发的45对EST-SSR引物中,38对引物有扩增产物,共扩增出660个条带,其中17对引物产生的多态性条带比率为100%,共扩增出多态性条带303条。96对SRAP通用引物中,67对引物有扩增产物,共扩增出1 347个条带,其中58对引物产生的多态性条带比率为100%,共扩增出多态性条带1 147条。种群遗传距离计算和聚类分析结果表明,西伯利亚蓼种群内和种群间均有遗传分化,分布区域相隔越远,遗传分化程度越高。结论：地理位置隔离、海拔高度均影响了国内西伯利亚蓼种质资源的分布和分化,人类活动可能也对其有影响。Objective： To explore the genetic differentiationof Polygonum sibiricum in different areas in China,in order toprovide basis for its population subdivision,evolution,protection and utilization,and quality control. Method： Fiftysix samples were collected from 13 sampling sites in four provinces of China. EST-SSR primers were developed and screened,and the genetic differentiation was evaluated with both EST-SSR and sequence related amplified polymorphism（ SRAP） markers. Result： Among 45 pairs of EST-SSR primers,38 pairs amplified 660 distinguished bands,and 17 pairs amplified 303 polymorphic bands,with a polymorphism rate of 100%. Among 96 pairs of SRAP primers,67 pairs amplified 1 347 distinguished bands,and 58 pairs amplified1 147 polymorphic bands,with a polymorphism rate of 100%. Genetic distance and cluster analysis revealed both inter-and inner-population have genetic differentiation. Conclusion： Genetic variation and geographic differentiation areobserved among P. sibiricum populations in China,which were affected by geographic distances,altitudes and possibly mankind activities.

关 键 词：相关序列扩增多态性标记 EST-SSR标记 遗传 分化 

分 类 号：R282.7[医药卫生—中药学] R29[医药卫生—中医学]