microRNA-1183通过抑制CBL-B的表达促进胃癌细胞的增殖及转移  

microRNA-1183 Promotes the Proliferation and Metastasis by Suppressing the Expression of CBL-B in Gastric Cancer Cells

作　　者：范一博[1] 车晓芳[1] 赵欢[2] 胡雪君[2] 曲秀娟[1] 刘云鹏[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤内科,沈阳110001 [2]中国医科大学附属第一医院呼吸疾病研究所老年病呼吸感染科,沈阳110001

出　　处：《中国医科大学学报》2018年第3期202-205,211,共5页Journal of China Medical University

基　　金：国家自然科学基金(81602098);辽宁省科技厅计划(2014226033);辽宁省科技厅中央引导地方科技发展专项资金计划(2016007010)

摘　　要：目的研究micro RNA-1183对胃癌细胞增殖及转移的影响,初步探讨micro RNA-1183和CBL-B信号转导通路在此过程中的作用。方法将人胃癌细胞MGC803瞬时转染micro RNA模拟物micro RNA-1183,采用实时PCR法检测胃癌细胞中micro RNA-1183的表达,MTT法检测细胞增殖的能力,Transwell法检测细胞的转移。双荧光素酶报告基因检测系统检测micro RNA-1183与CBL-B的结合,Western blotting观察蛋白的表达。结果 MTT法显示micro RNA-1183可促进胃癌细胞MGC803的增殖,Transwell法显示micro RNA-118促进了胃癌细胞MGC803的转移。BLAST对比分析结果显示,CBL-B是micro RNA-1183的靶基因之一。Western blotting结果显示,过表达micro RNA-1183后,CBL-B的表达明显下调。双荧光素酶报告基因检测系统证实CBL-B是micro RNA-1183的靶基因。敲除CBL-B后,促进了MGC803的增殖与转移。micro RNA-1183通过抑制CBL-B促进了胃癌细胞MGC803的增殖与转移。结论 micro RNA-1183通过抑制CBL-B的表达,促进胃癌细胞MGC803的增殖及转移。Objective To investigate the effect of micro RNA-1183 on proliferation and metastasis on gastric cancer cells and to explore the role of micro RNA-1183 and CBL-B signaling pathways in this process. Methods MGC803 cells were transfected with a micro RNA-1183 mimic. Real-time PCR detected the expression of micro RNA-1183 in gastric cancer cell line MGC803. MTT detected the proliferative effect of micro RNA-1183 on MGC803 gastric cancer cells. A Transwell assay detected the effect of micro RNA-1183 on the metastasis of MGC803 gastric cancer cells. A dual luciferase reporter assay detected the binding ability between micro RNA-1183 and CBL-B. The expression of the protein was tested by Western blotting. Results MTT assay results showed that micro RNA-1183 promoted the proliferation of MGC803 cells. Transwell assay results revealed that micro RNA-1183 promoted the metastasis of MGC803 cells. The results of BLAST contrast analysis show that CBL-B is one of the target genes of micro RNA-1183. Western blotting analysis showed that the mimic micro RNA-1183 inhibited the expression of CBL-B. A dual luciferase reporter assay showed that CBL-B was the target gene of micro RNA-1183. A CBL-B knockdown promoted the proliferation and metastasis of MGC803 cells. micro RNA-1183 promoted the proliferation and metastasis of MGC803 cells by inhibiting the expression of CBL-B. Conclusion micro RNA-1183 can inhibit the proliferation and metastasis of gastric cancer cell lines by inhibiting the expression of CBL-B.

关 键 词：胃癌 microRNA-1183 CBL-B 增殖 转移 

分 类 号：R735[医药卫生—肿瘤]