Mdivi-1对Aβ致伤大鼠海马区神经元线粒体凋亡率及drp-1表达的影响  

Effects of Mdivi-1 on mitochondrial apoptosis rate and Drp-1 expression in rat hippocampal neurons injured by Aβ

作　　者：任秀敏 于佳楠 宋红梅 姜尧佳 赵长海[2] 

机构地区：[1]佳木斯大学附属第一医院 [2]佳木斯市中心医院,黑龙江佳木斯154002

出　　处：《微量元素与健康研究》2018年第2期1-2,5,共3页Studies of Trace Elements and Health

基　　金：黑龙江省卫生计生委科研课题(编号:2016-306)

摘　　要：目的:观察线粒体分裂抑制剂(Mdivi-1)对Aβ致伤离体培养的大鼠海马神经元线粒体的凋亡率及线粒体动力相关蛋白1(drp-1)表达的影响。方法:选择出生年龄小于24 h的大鼠,取其海马神经元进行原代培养。将培养的海马神经元细胞分成4组,正常组不给予任何处理,Aβ组在细胞培养基中加入Aβ毒素,Mdivi-1组为在细胞培养基中加入Mdivi-1,Aβ+Mdivi-1组为在细胞培养基中加入Aβ和Mdivi-1。采用MTT比色法检测细胞凋亡率,Western blot技术检测drp-1的表达。结果:MTT检测结果显示与正常组比较Aβ组的海马神经元凋亡率明显上升,差异有统计学意义(P<0.05),与Aβ组比较,Aβ+Mdivi-1组的海马神经元凋亡率下降,差异有统计学意义(P<0.05);WB检测结果显示与正常组比较,Aβ组的drp-1表达上调,差异有统计学意义(P<0.05),Aβ+Mdivi-1组较Aβ组表达下调,差异有统计学意义(P<0.05)。结论:mdivi-1能抑制Aβ毒素作用,从而减少神经元细胞线粒体的调亡,drp-1表达的减少也能稳固线粒体的分裂。因此,对Aβ和drp-1的定向干预治疗可能会成为治疗AD的关键靶点。Objective：To observe the effect of mitochondrial fission inhibitor（Mdivi-1） on the mitochondrial apoptosis rate and the expression of mitochondrial dynamic associated protein1（Drp-1） in vitro cultured rat hippocampal neurons injured by Aβ.Methods：The hippocampal neurons of rats were selected for primary culture at the age of less than 24 h.The cultured hippocampal neurons were divided into four groups,the normal group received no treatment,Aβ group in the beta cell culture medium added Aβ toxin,in group Mdivi-1,Mdivi-1 were added into the culture medium,and Aβ ＋ Mdivi-1 group in the cell culture medium added Aβ and Mdivi-1.Finally,MTT colorimetric assay was used to detect the apoptosis rate and the expression of Drp-1 was detected by Western blot.Results：MTT results showed that compared with the normal group,the hippocampal neuronal apoptosis rate in Aβgroup was significantly increased,the difference was statistically significant（P 0.05）,while compared with group Aβ,the apoptosis rate of hippocampal neurons in Aβ ＋ Mdivi-1 group decreased,the difference was statistically significant（P 0.05）; WB test results showed that compared with the normal group,the expression of Drp-1 in the A group was up-regulated,and the difference was statistically significant（P 0.05）,and compared with the Aβgroup,the expression of Drp-1 in group Aβ ＋ Mdivi-1 was down-regulated,the difference was statistically significant（P 0.05）.Conclusion：mdivi-1 can inhibit the effect of Aβtoxin,not only reduces neuronal cell mitochondrial apoptosis,thus reducing neuronal cell mitochondrial apoptosis,decreasing the expression of Drp-1 can also stabilize mitochondrial fission.Therefore,targeted intervention on Aβ and Drp-1 may become a key target in the treatment of AD.

关 键 词：Mdivi-1 AΒ 海马神经元 p-drp-1 

分 类 号：R749.1[医药卫生—神经病学与精神病学]