FAM96B在胃癌组织中的表达及其对胃癌细胞增殖凋亡和侵袭转移的影响  

Expression of FAM96B in gastric carcinoma and its effect on proliferation and apoptosis of gastric cancer cells

作　　者：叶家欣[1] 王世杰 马丹丹 蔡逊[1] 张智勇[1] 张建新[1] 田俊[2] Ye Jiaxin, Wang Shijie, Ma Dandan, et al(Dept of General Surgery, Wuhan General Hospital of Chinese People＇ s Liberation Army, Wuhan 43007)

机构地区：[1]中国人民解放军武汉总医院普通外科,武汉430070 [2]华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉430030

出　　处：《安徽医科大学学报》2018年第3期364-367,共4页Acta Universitatis Medicinalis Anhui

基　　金：湖北省自然科学基金(编号:2012FFB06805);国家自然科学基金(编号:81350006)

摘　　要：目的观察96序列相似的家庭成员B(FAM96B)在80例胃癌组织中的表达,分析表达差异与胃癌生物学特征的关系,并探讨FAM96B对胃癌细胞系SGC7901细胞增殖及凋亡的影响。方法采用Western blot法检测FAM96B蛋白在80例胃癌组织及配对癌旁组织中的表达,并统计分析FAM96B蛋白表达差异与胃癌临床病理特征之间的相关性。采用脂质体转染法将含人FAM96B全长序列的pcD NA3.1-myc-FAM96B重组质粒转染至SGC7901细胞中,分别采用MTT法及流式细胞术检测FAM96B过表达对SGC7901增殖和凋亡的影响。结果 Western blot结果显示FAM96B在癌旁组织中的蛋白表达水平显著高于胃癌组织(t=25.218,P<0.05)。胃癌组织中FAM96B表达水平与胃癌浸润深度、淋巴结转移、远处转移及TNM分期显著相关(P<0.05),然而与患者性别、年龄、肿瘤大小无关。MTT细胞实验结果表明:FAM96B组吸光度值显著低于对照组(P<0.05)。细胞凋亡检测结果示:过表达FAM96B组的细胞凋亡率高于对照组(t=85.585,P<0.05)。结论 FAM96B在人胃癌组织中表达显著下降,其表达水平与胃癌的发生发展、侵袭转移有密切相关。过表达FAM96B可抑制胃癌细胞增殖且诱导其凋亡。Objective To investigate the expression of family with sequence similarity 96, member B （FAM96B） in 80 cases of gastric cancer and explore the correlation between FAM96B expression and its clinical biological characteristics of gastric cancer. Then to evaluate the proliferation and apoptosis effect of FAM96B overxpression on human gastric carcinoma cell line SGC7901. Methods Western blot was employed to detect FAM96B expression in 80 cases of gastric cancer tissues and its adjacent normal tissues. Furthermore, the correlation between FAM96B expression levels and elinicopathological characteristics of ganstric cancer was analyzed. Moreover, the recombinant plasmid of pcDNA3.1-myc-FAM96B containing the whole sequence of human FAM96B gene was constructed and transfected into SGC7901 cells. MTF essay was used to evaluate the proliferation of transfected cells, and apoptosis was detected by flow cytometry. Results Western blot results showed that FAM96B protein expression levels was significantly higher in the adjacent normal tissues than in gastric cancer tissues （t =25.218, P 〈 0. 05 ）. And the FAM96B protein expression was tightly correlated with depth of infiltration, lymph nodes metastasis, distant metas- tasis and TNM stage （P 〈 0. 05）, but not with gender, age, tumor size. The MTT assay showed that the a value in FAM96B overexpression group was significantly lower than in control group （ P 〈 0. 05 ）. FACS analysis indicated that the cell apoptotic rate was increased in FAM96B group than in the control group （ t = 85. 585, P 〈 0.05 ）. Conclusion The FAM96B protein expression is significantly decreased in gastric cancer tissues as compared with its adjacent normal tissues. In addition, FAM96B may be a good biomarker to evaluate the development, invasion and metastasis of gastric cancer. Overexpression of FAM96B can inhibit the proliferation and induce the apoptosis of SGC7901 cells.

关 键 词：96序列相似的家庭成员B 胃癌 表达 增殖 凋亡 侵袭转移 

分 类 号：R735.2[医药卫生—肿瘤]