动物源性食品中56种兽药残留物的UPLC-MS/MS高通量分析  

UPLC-MS/MS high-throughput detection of 56 veterinary drug residues in food products of animal origin

作　　者：王嘉琦[1] 马立利[1] 曹英华[1] 王建凤[1] 刘喆[1] 范筱京[1] 贾丽[1] 

机构地区：[1]北京市理化分析测试中心,北京100089

出　　处：《分析试验室》2018年第3期306-310,共5页Chinese Journal of Analysis Laboratory

基　　金：北京市科学技术研究院2016年青年骨干计划项目(201616)资助

摘　　要：建立了超高效液相色谱串联质谱法同时测定56种动物源性兽药残留物的分析方法。以乙腈-0.5%乙酸水溶液(85:15,V/V)为提取溶剂,采用固相萃取柱(Oasis PRi ME HLB)进行样品净化,通过UPLC-MS/MS分析测定,基质曲线外标法定量。方法定量限为1.0μg/kg(喹诺酮类)、1.5μg/kg(糖皮质类)、10μg/kg(磺胺类)、1.0μg/kg(β-受体激动剂类)。56种兽药残留物在牛肉、猪肉、鸡肉和奶等基质样品中回收率范围为63.2%~101.5%,基质标准工作曲线线性相关系数大于0.937,精密度RSD<15%(n=3)。方法适用于动物源性食品中兽药残留物的分析。A method for simultaneous determination of 56 veterinary drug residues in food products of animal originwas established by UPLC-MS/MS.After extraction by the mixed solvent of acetonitrile and0.5% acetic acid in water（85： 15）,the sample extracts were subjected to a cleanup procedure by using Oasis PRi ME HLB column.The residue contentswas analyzed by UPLC-MS/MS,and the quantitative information was obtained bymatrix-matched calibration with external standard.The LOQs of 4-quinolones,glucocorticoid,sulfonamides and β-agonist were determined to be 1.0,1.5,10 and1.0 μg/kg,respectively.56 veterinary drugs exhibited good linear relationship with correlation coefficient of 0.937 and RSD less than 15%（n = 3）.The recoveries of veterinary drugs in matrix including beef,pork,chicken and milk ranged from 63% to 101%.The method was quick and simple,and would be suitable for the detection of veterinary drug residues in food products of animal origin.

关 键 词：兽药 超高效液相色谱串联质谱 固相萃取柱Oasis PRIME HLB 

分 类 号：TS207.5[轻工技术与工程—食品科学]