大鼠面神经钳夹损伤后蛋白质组学研究  

Study on proteomics of rats with facial nerve clamp injury

作　　者：李伯翰 鄂玲玲[2] 谢潇[3] 刘洪臣 

机构地区：[1]北京大学第三医院口腔科,医师北京100142 [2]解放军总医院口腔医学研究所,博士后北京100853 [3]空军司令部门诊部口腔科,主治医师北京100843

出　　处：《中华老年口腔医学杂志》2017年第6期328-331,343,共5页Chinese Journal of Geriatric Dentistry

基　　金：国家自然科学基金(项目编号:81400528);中国博士后科学基金(项目编号:20130390827)

摘　　要：目的:通过蛋白质组学研究,检测面神经钳夹损伤后的大鼠面神经与正常大鼠面神经的差异蛋白。方法:分别取正常大鼠面神经和钳夹损伤30秒后的大鼠面神经,分别作为对照组和实验组。提取两组大鼠面神经蛋白后,质谱分析并鉴定筛选出2组大鼠面神经组织差异表达的蛋白。采用SPSS15.0软件包对数据进行统计学分析。结果:经蛋白质组学研究,成功鉴定出19个2组间显著性差异蛋白,其中4个蛋白点差异倍数在20倍以上。4个蛋白主要涉及代谢、信息储存以及信号转导3大功能。结论:面神经钳夹损伤后与正常大鼠面神经组织间存在差异蛋白,有助于探讨面神经损伤后的蛋白调控机制。Objective： To investigate the differentially expressed proteins of facial nerve between normal rats and facial nerve crush injury rats. Methods： Facial nerve and crush injury nerve were selected as normal controls control group and experimental group respectively. After protein extraction, differentially expressed proteins were separated by two-dimensional gel electrophoresis （2-DE） and identified by MALDI-TOF/TOF. Statistical analysis of data was performed using SPSS 15.0 software package. Results： According to the proteomics study, there were 19 significant differences between the two groups, and the difference of 4 protein points was more than 20 times. The 4 proteins fell into 3 functional categories as follows： metabolism, information storage and transduction. Conclusion： The differentially expressed proteins of facial nerve between normal rats and facial nerve injury rats may be new targets for investigating the mechanisms of the effect of nerve injury.

关 键 词：面神经损伤 蛋白质组学 差异蛋白 

分 类 号：R782[医药卫生—口腔医学]