高温与性激素诱导对褐牙鲆kiss2和gpr54-2基因表达的影响  

Effects of High Temperature and Sex Hormone on Expression of Kiss2 and gpr54-2 Genes in Olive Flounder Paralichthys olivaceus

作　　者：向玉婷 张俊玲[1] 施志仪[1] 

机构地区：[1]上海海洋大学水产与生命学院,农业部淡水水产种质资源重点实验室,上海201306

出　　处：《水产科学》2018年第2期227-232,共6页Fisheries Science

基　　金：国家自然科学基金青年基金资助项目(41306128)

摘　　要：Kisspeptin是调控脊椎动物生殖的重要神经内分泌因子,本实验室先前已鉴定了褐牙鲆kiss2及其受体gpr54-2基因,二者在褐牙鲆脑和性腺中具有较高表达,但其在褐牙鲆早期性腺分化中的作用以及温度、外源激素对其基因表达的影响尚不清楚。因此本研究设置高温(28℃)、雌激素(10μg/L)、雄激素(10μg/L)处理组和常温(20℃)对照组培育性腺分化早期的褐牙鲆幼鱼(45日龄、55日龄和65日龄),利用荧光定量PCR技术分析了高温和性激素诱导对褐牙鲆早期性腺分化过程中kiss2及其受体gpr54-2基因表达的影响。试验结果显示,高温和雌激素处理可以促进褐牙鲆脑中kiss2和性腺中gpr54-2的表达,却下调了褐牙鲆性腺中kiss2的表达;而雄激素处理则使褐牙鲆脑和性腺中kiss2和gpr54-2的表达几乎都受到显著的抑制作用。本研究结果为进一步阐述kiss2和gpr54-2基因在褐牙鲆早期性腺分化中的作用奠定基础。Kisspeptin is an important neuroendocrine factor for the regulation of vertebrate reproduction.The kiss2 and its receptor（gpr54-2）cDNAs have been identified previously and are highly expressed in the brain and gonads of olive flounder Paralichthys olivaceus.However,the function of kiss2 and gpr54-2 in the early gonadal differentiation and the effects of temperature and exogenous hormones on the genes expression are unclear.Juvenile flounders（45 days post-hatch dph,55 dph and 65 dph）were cultured in four experimental groups of high temperature（28 ℃）,estrogen（10μg/L）,androgen（10μg/L）and normal temperature（20 ℃）,and the effects of high temperature and sex hormone on the expression of kiss2 and its receptor gpr54-2 were analyzed by qPCR.The results showed that the high temperature and estrogen treatment led to promote the expression of kiss2 in brain and gpr54-2 in gonad of olive flounder,but downregulated the expression of kiss2 in gonad of the flounder,while the androgen treatment almost significantly inhibited the kiss2 and gpr54-2 expression.The findings provide a basis for further understanding of the role of kiss2 and gpr54-2 genes in the early gonadal differentiation in olive flounder.

关 键 词：褐牙鲆 kiss2 gpr54-2 高温 性激素 

分 类 号：Q786[生物学—分子生物学]