超高效液相色谱法测定清咽含片中罗汉果皂苷V  

Determination of saponin V from Siraitia grosvenorii in Chinese pharynx tablets by ultra performance liquid chromatography

作　　者：蔡伟江 魏鲜娥 陈珊珊 林丽 CAI Wei-Jiang, WEI Xian-E, CHEN Shan-Shan, LIN Li(By-Health Co., Ltd., Zhuhai 519040, Chin)

机构地区：[1]汤臣倍健股份有限公司,珠海519040

出　　处：《食品安全质量检测学报》2018年第5期1017-1020,共4页Journal of Food Safety and Quality

摘　　要：目的建立超高效液相色谱法(ultra performance liquid chromatography,UPLC)测定清咽含片中罗汉果皂苷V含量的方法。方法色谱条件为色谱柱:XSELECT HSS T_3(100 mm×2.1 mm,2.5μm);流动相:乙腈:0.1%磷酸水溶液,梯度洗脱,流速:0.4 mL/min;柱温:40℃;进样量:5μL;检测波长:203 nm。结果结果表明,罗汉果皂苷V线性范围为0.033~0.333 mg/mL(r=1.0000),检出限66.4μg/g,定量限221.6μg/g,精密度(relative standard deviation,RSD)为1.5%,平均回收率97.6%(n=9)。结论此法操作简单、准确、快速,可作为清咽含片中罗汉果皂苷V含量的检测方法。Objective To establish a method for determination of the content of saponin V from Siraitia grosvenorii in Chinese pharynx tablets by ultra performance liquid chromatography （UPLC）. Methods The chromatographic conditions were as follows： chromatographic column was XSELECT HSS T3 （100 mm×2.1 mm, 2.5 μm）, flow phase was acetonitrile： 0.1% phosphoric acid water with gradient elution, flow rate was 0.4 mL/min, column temperature was 40 ℃, sample amount was 5 L, and detection wavelength was 203 nm. Results The results showed that the linear range of saponin V was 0.033~0.333 mg/mL （r=1.0000）. The limit of detection was 66.4 g/g, and the limit of quantitative was 221.6 g/g, the relative standard deviation （RSD） was 1.5%, and the average recovery rate was 97.6% （n=9）. Conclusion This method is simple, accurate and rapid, which can be used for determination of the content of saponin from Siraitia grosvenorii in Chinese pharynx tablets.

关 键 词：罗汉果皂苷V 超高效液相色谱法 清咽含片 

分 类 号：O657.72[理学—分析化学] R286.0[理学—化学]