干旱胁迫下外源激素对大豆叶片表皮透性及蜡质微形态影响  

Effects of Exogenous Hormones on Cuticular Permeability and Wax Micromorphology of Soybean Leaves under Drought Stress

作　　者：王丽娜[1] 金勋[1] 杨柳[1] 潘红丽[1] 芮海英[1] 李娜[1] 李泽宇[1] 齐国超[1] WANG Li-na, JIN Xun, YANG Liu, PAN Hong-li, RUI Hai-ying, LI Na, LI Ze-yu, QI Guo-chao(Daqing Branch of Heilongjiang Academy of Agricultural Sciences, Daqing 163316, Chin)

机构地区：[1]黑龙江省农业科学院大庆分院,黑龙江大庆163316

出　　处：《大豆科学》2018年第2期246-250,共5页Soybean Science

摘　　要：利用外源激素ABA、ETH处理大豆幼苗,通过人工控水研究外源激素喷施对大豆生理及叶片表皮蜡质微形态的影响。结果表明:当ABA、ETH处理14 d时,各处理之间叶片的相对含水量、叶绿素含量无明显差异,叶片失水率则表现为当离体叶片失水80 min后ABA、ETH处理低于CK。当ABA、ETH处理28 d时,各处理相对含水量明显高于14d,ABA处理与ETH、CK处理之间叶片相对含水量差异显著,ETH与CK处理之间叶片相对含水量差异不显著;各处理的叶绿素含量低于14 d的含量,ABA处理的叶片叶绿素含量与ETH处理差异显著,与CK处理差异不显著;各处理叶片的失水率不断增加,当离体叶片失水60 min时ABA、ETH处理的叶片失水率趋于相同,显著的高于CK处理。ABA、ETH、CK处理28 d,扫描电镜观察大豆叶片表皮蜡质呈棒条状,处理之间数量无明显差异,而ETH处理的茎秆表皮的蜡质数量明显少于ABA、CK处理,呈片状结构。The effects of exogenous hormone ABA and ETH spraying on soybean physiology and leaf epidermal wax morpholo- gy were studied by artificial water control, which laid the foundation for later study on physiological and molecular mechanisms of drought resistance in soybean. The results showed that ABA and ETH were treated with 14 d, the relative water content and chlorophyll content of leaves were not signifieantly different among the treatments, the water loss rate of ABA and ETH treated leaves were lower than CK after 80 min of water loss. On 28d, the relative water content of hedehydrated leaves in vitro ABA, ETH were significantly higher than that of 14 d, the relative leaf water content of ABA was significandy between ETH and CK treatment, there was no signifieant difference between ETH and CK; the chlorophyll content was lower than the content of 14 d, the leaf ehlorophyll content of ABA treatment and ETH treatment were signifieant difference, ABA treatment and CK treat- ment had no significant differenee; the leaf water loss rate of each treatment was progressively increased, after 60 min the rate of ABA and ETH tended to be the same. The ABA, ETH and CK treatments of 28 d, scanning electron microscope observa- tion of soybean leaf epidermis wax was stick bar with no significant difference in number, and stem epidermis wax number of ETH treatment was significantly less than ABA and CK treatment, presented sheet structure.

关 键 词：相对含水量 失水率 叶绿素 表皮蜡质数量 

分 类 号：S565.1[农业科学—作物学]