MTA3在肝细胞癌中的表达及意义  

Expression of MTA3 in Hepatocellular Carcinoma and Its Significance

作　　者：周薏[1] 傅志泉[1] 朱樑[2] ZHOU Yi, FU Zhiquan, ZHU Liang.(Shanghai Hospital of Integrated Traditional Chinese with Western Medicine, Shanghai, 20008)

机构地区：[1]上海市中西医结合医院,200082 [2]第二军医大学附属长征医院,200003

出　　处：《实用癌症杂志》2018年第4期530-533,共4页The Practical Journal of Cancer

基　　金：上海市自然基金项目(编号:16ZR1433800)

摘　　要：目的探讨肝细胞癌组织及肝癌细胞中MTA3的表达情况及其对肝癌细胞增殖的影响。方法采集临床肝细胞癌样本,应用定量PCR和免疫组织化学方法,检测MTA3的表达,同时在多种人肝癌细胞株中应用定量PCR检测MTA3的表达;使用MTA3的干扰siRNA转染人肝癌细胞株HepG2和Huh7,分别采用CCK-8实验和克隆形成实验检测下调MTA3表达对人肝癌细胞增殖的影响。结果 MTA3在肝细胞癌组织中表达水平显著升高(P<0.05),在肝细胞癌组织中其阳性表达率显著高于癌旁非癌组织(P<0.01),人肝癌细胞株HepG2和Huh7中的MTA3表达水平显著高于正常肝细胞株L02(P<0.01),构建两个MTA3的si RNA干扰效率均在50%左右,转染siRNA-MTA3后,人肝癌细胞株HepG2和Huh7的增殖受到显著抑制(P<0.05)。结论 MTA3作为促癌基因参与了肝细胞癌的发生,成功构建了MTA3的干扰siRNA,干扰MTA3能有效抑制肝癌细胞的增殖,MTA3有望成为肝细胞癌靶向治疗的新靶点。Objective To investigate the expression and significance of MTA3 in HCC. Methods The expression of MTA3 in HCC liver tissue was preliminary screened through onco mine database. The expression of MTA3 in cancerous tissue and para-cancer noncancerous tissue was deter mined by qPCR and immunohistochemistry. The expression of MTA3 in human liv- er cancer cell lines was detected by qPCR. MTA3 targeted siRNA was transfected into human liver cancer cell lines HepG2 and HuhT. Cell proliferation was analyzed by CCK-8 assay and clone formation assay. Results The expression level of MTA3 was in- creased in HCC liver cancer （ P 〈 0.05 ）. Expression level of MTA3 in cancerous tissue was significantly higher than in para- cancer noncancerous tissue （ P 〈 0.01 ）. Expression level of MTA3 was consistently increased in human liver cancer cell lines HepG2 and Huh7 compared with normal liver cell L02 （P 〈 0.01 ）. Compares with cells transfected with control siRNA, HepG2 and Huh7 which transfected with MTA3 siRNA showed markedly repressed cell proliferation. Conclusion MTA3 as a cancer promoting gene is involved in the pathogenesis of HCC. Suppression of MTA3 can significantly repress human liver cancer cell lines proliferation. Therefore, MTA3 may become a potential target for treatment of HCC.

关 键 词：肝细胞癌 MTA3 SIRNA 增殖 基因治疗 

分 类 号：R735.7[医药卫生—肿瘤]