云南汉族人群NKG2D基因多态性与宫颈癌发生发展的相关性  

Association of Polymorphisms in NKG2D Gene with Initiation and Development of Cervical Cancer in Yunnan Han Population

作　　者：杨晓蕾[1] 戴书颖 王小娜[1] 张新文[1] 刘舒媛[1] 付杰 牛步青 杨欢 唐军 王培智[1] 李传印[1] YANG Xiaolei1 DAI Shuying2, WANG Xiaona1 ,ZHANG Xinwen1 ,LIU Shuyuan1, FU Jie1 , NIU Buqing1, YANG Huan1 , TANG Jun1 , WANG Peizhi1 , LI Chuanyin1(1. Institute of Medical Biology, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Yunnan key Laboratory of Vaccine Development for Major Infectious Diseases, Kunming 650118, Yunnan, China; 2. School of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, Chin)

机构地区：[1]中国医学科学院&北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室,云南昆明650118 [2]昆明医科大学基础医学院,云南昆明650500

出　　处：《贵州医科大学学报》2018年第3期259-263,304,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金项目(81573206);云南省应用基础研究重点项目(2016FA034);云南省应用基础研究-昆医联合专项(2017FE467-012)

摘　　要：目的:探讨云南汉族人群NKG2D(member D)基因中的单核苷酸多态性(SNP)位点rs2255336(T>C)及rs2617160(A>T)的多态性与宫颈癌的相关性。方法:选取云南地区汉族宫颈上皮内瘤变(CIN)患者136例作为CIN组、227例宫颈癌患者作为癌症组及483例健康体检人群作为对照组,采用Taqman基因分型法对3组受检者NKG2D基因中2个SNP位点rs2255336(T>C)、rs2617160(A>T)进行基因分型,研究rs2255336(T>C)、rs2617160(A>T)位点的等位基因、基因型及所构建的单倍型在各组中分布频率,用遗传模式分析软件SNPStats分析2个SNP位点的基因型与宫颈癌的相关性。结果:对照组与CIN组NKG2D基因中rs2617160(A>T)等位基因C和T分布频率比较,差异有统计学意义(P=0.049),该位点等位基因A可能是云南汉族人群CIN发生的风险因素(OR=1.31,95%CI为1.00~1.72),而该位点在对照组与癌症组、CIN组与癌症组中的分布频率比较差异无统计学意义(P>0.05);recessive模式为CIN组与对照组分析的最优遗传模式,该模式下TT-AT基因型相对于AA基因型是CIN发生的保护性因素(OR=0.58,95%CI为0.37~0.92);rs2255336(T>C)位点等位基因和基因型在对照组、CIN组、癌症组间的分布频率比较,差异无统计学意义(P>0.05)。结论:NKG2D基因中多态性位点rs2617160(A>T)的等位基因A可能是云南汉族人群宫颈癌发生的风险因素。Objective： To investigate the association of the single nucleotide polymorphisms （SNPs） （ rs2255336 T 〉 C and rs2617160 A 〉 T） in NKG2D （ natural-killer group 2, member D） gene with the initiation and progression of cervical cancer in Yunnan Han population. Methods： A total of 136 patients with cervix intraepithelial neoplasia （ CIN）, 227 patients with cervical cancer and 483 healthy individuals were recruited in the study, and the SNPs were genotyped by Taqman assay. Moreover, the haplotypes were constructed and the association of the haplotypes with cervical cancer was analyzed. Results ： The results showed that the allelic frequencies of rs2617160 A 〉 T were significantly different between CIN and control groups （P =0. 049, OR = 1.31, 95% CI = 1.00 - 1.72）. However, there was no significant association between control and cancer groups, and between CIN and cancer groups （P 〉 0.05）. The inheritance analysis showed that the best fit inheritance model of rs2617160 was recessive model in the comparison of CIN and Control groups. Compared to AA genotype, TY-AT was a protective factor of the initiation of cervical cancer（ OR = 0.58, 95% CI = 0.37 -0. 92）. Additionally, there was no significant difference of the allelic and genotypic frequencies of rs2255336 T 〉 Camong the three groups （ P 〉 0.05 ）. Conclusion ： The A allele of rs2617160 A 〉 T may be a risk factor of initiation of the cervical cancer.

关 键 词：多态性 单核苷酸 NKG2D 宫颈肿瘤 宫颈上皮内瘤变 基因型 云南 汉族 相关性 

分 类 号：R737.33[医药卫生—肿瘤]