长链非编码RNAMALAT1在口腔鳞状细胞癌患者血浆中的表达及意义  

Expression and clinical significance of plasma long non-coding RNA MALAT1 in patients with oral squamous cell carcinoma

作　　者：王军[1] 赵思语 欧阳少波[2] 廖岚[2] WANG Jun, ZHAO Siyu2, OUYANG Shaobo2 , LIAO Lan2(1. 330006, Department of Oral and Maxillofacial Sur- gery, the Second Affiliated Hospital of Nanchang University, China; 2. Department of Oral Prosthodontics, Affiliated Stomatological Hospital of Nanchang University ＆ Jiangxi Provinial Key Laboratory of Oral Biomedicin)

机构地区：[1]南昌大学第二附属医院口腔颌面外科,330006 [2]南昌大学附属口腔医院修复科,江西省口腔生物学重点实验室

出　　处：《实用口腔医学杂志》2018年第2期188-192,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号：81660444);江西省自然科学基金(编号：20171BAB205050);江西省重点研发计划项目(编号：20161BBG70149)

摘　　要：目的:探讨长链非编码RNA肺腺癌转移相关转录本1(MALAT1)在口腔鳞癌患者血浆中的表达及临床意义。方法:纳入70例口腔鳞癌患者,50例健康对照者;采用实时荧光定量PCR的方法检测2组受试者血浆中MALAT1的表达。分析15例口腔鳞癌患者手术前后血浆中MALAT1的表达变化。结果:口腔鳞癌患者血浆MALAT1的表达水平显著高于健康对照组(P<0.001),且与患者TNM分期、肿瘤分化程度及淋巴结转移显著相关(P<0.05)。患者术后血浆MALAT1表达显著降低(P<0.001)。血浆MALAT1诊断口腔鳞癌的曲线下面积(AUC)为0.814,敏感度和特异度分别为87.43%和72.00%。结论:血浆MALAT1有望作为口腔鳞癌辅助诊断指标。Objective： To investigate the expression of long non-coding RNA （lncRNA） metastasis-associated lung adenocarcinoma transcript 1 （ MALAT1 ） in plasma of patients with oral squamous cell carcinoma （OSCC） and its clinical significance. Methods ： 70 OSCC patients and 50 healthy controls were included. The relative expression of MALAT1 in plasma was examined by quantitative real- time PCR. The expression of MALAT1 in plasma in 15 OSCC patients was analyzed retrospectively 30 days after operation. Results： The expression level of MALAT1 in plasma of OSCC patients was significantly higher than that of healthy controls （P 〈 0. 001 ）. The ex- pression level of MALAT1 in OSCC patients was significantly correlated with TNM stage, tumor differentiation and lymph node metasta- sis（ P 〈 0.05 ）. After operation the expression level of MALAT1 in plasma of OSCC patients was significantly decreased（ P 〈 0.001 ）. The AUC of the diagnosis of OSCC with MALAT1 was 0. 814, and the sensitivity and specificity were 87.43% and 72.00% respective- ly. Conclusion： MALAT1 can be used as an auxiliary diagnostic marker for OSCC.

关 键 词：口腔鳞状细胞癌(OSCC) 长链非编码RNA(1ncRNA) 肺腺癌转移相关转录本1(MALAT1) 生物标志物 

分 类 号：R739.8[医药卫生—肿瘤]