IL-1β激活p38 MAPK通路促进人颞下颌关节滑液间充质干细胞分泌IL-6和IL-8  

p38 MAPK pathway promotes IL-6 and IL-8 secretion of synovial fluid mesenchymal stem cells from temporomandibular joint inflamed by IL-1β

作　　者：刘文静 孙养鹏 郑有华[2] 王周涛 张志光[2] LIU Wenjing1, SUN Yangpeng2, ZHENG Youhua2, WANG Zhoutao2, ZHANG Zhiguang2(1. 510280 Guangzhou, Department of Prosthodontics, Stomatological Hospital, Southern Medical University, China. 2. Department of Oral and MaxiUofacial Surgery, Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Key Laboratory of Stomatology, Sun Yat- sen University, Guangzho)

机构地区：[1]南方医科大学口腔医院修复科,广东省广州市510280 [2]中山大学光华口腔医学院·附属口腔医院口腔颌面外科,广东省口腔医学重点实验室

出　　处：《实用口腔医学杂志》2018年第2期215-219,共5页Journal of Practical Stomatology

摘　　要：目的:探讨p38 MAPK信号通路在IL-1β介导下人颞下颌关节滑液间充质干细胞(synovial fluid derived mesenchymal stem cells,SFMSCs)炎性因子分泌中的作用。方法:体外培养SFMSCs,分为对照组和IL-1β(10 ng/ml 2 h)处理组,Western blot检测p38 MAPK蛋白表达,RT-PCR和炎症细胞因子CBA检测对照组、IL-1β处理组和p38 MAPK抑制剂组(SB-203580 10μmol/L、1 h后加入IL-1β10 ng/ml 2 h)h SFMSCs及培养基中IL-6和IL-8的表达和分泌。结果:IL-1β处理组SFMSCs p-p38MAPK蛋白表达、IL-6和IL-8的基因表达及分泌量均明显增加,而p38 MAPK抑制剂组则均明显减少。结论:p38 MAPK信号通路参与炎性微环境下人SFMSCs的炎性分泌。Objective： To investigate the role of p38 MAPK pathway in IL-1β induced inflammatory secretory properties mesenchymal stem cells（SFMSCs） from human synovial fluid of temporomandibular joint. Methods： In vitro cultured bSFMSCs were divided into control group and IL-1β treated group（ 10 ng/ml IL-1β for 2 h） , the activation of p38 MAPK was examined with western blot. Then, the gene expression and the secretion of IL-6 and IL-8 in control group, IL-1β treated group and p38 MAPK inhibitor pre-treated group （pretreatment with SB-203580 10μmo]/L prior to the addition of 10 ng/ml IL-1β）were examined by RT-PER and cytometrie bead array（CBA）. Results： It was observed that the protein level of p-p38 MAPK, the secretion of IL-8 and IL-6 were increased in IL-1β treated group, and suppressed in p38 MAPK inhibitor pre-treated group. Conclusion： p38 MAPK pathway of SFMSCs might play a role in IL-6 and IL-8 secretion by SFMSCs in inflammatory environment.

关 键 词：颞下颌关节 滑液间充质干细胞 细胞因子 丝裂原活化蛋白激酶(MAPK) 

分 类 号：R782.6[医药卫生—口腔医学]