凉粉草化学成分分离鉴定  

Isolation and Identification of Chemical Constituents of Mesona chinensis

作　　者：黄艳萍[1] 宋家玲[2] 吴继平[1] 袁萍[1] 沈晓萌[1] 邱声祥[2] HUANG Yan-ping1 , SONG Jia-ling2 , WU Ji-ping1 , YUAN Ping1 , SHEN Xiao-meng1 , QIU Sheng-xiang2.(1. Research ＆ Development Center for Engineering and Technology of Medicinal Resources Protection and Utilization in South China, Guangdong Food and Drug Vocational College, Guangzhou 510520, China; 2. Key Laboratory for Sustainable Utilization of Plant Resources, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Chin)

机构地区：[1]广东食品药品职业学院南药资源保护与利用工程技术开发研究中心,广州510520 [2]中国科学院华南植物园植物资源可持续利用重点实验室,广州510650

出　　处：《中国实验方剂学杂志》2018年第6期77-81,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技计划项目(2011B020301007,2013B020311013)

摘　　要：目的：研究唇形科（Labiatae）植物凉粉草Mesona chinensis的干燥地上部分的化学成分,从物质基础的角度探寻其作为药食同源的可能性。方法：取凉粉草的干燥地上部分,用95%乙醇浸泡提取,经减压浓缩后得到凉粉草浸膏,然后将凉粉草浸膏加水溶解后,依次用正己烷,乙酸乙酯溶剂萃取,得到正己烷部位浸膏和乙酸乙酯部位浸膏,然后采用硅胶、小孔树脂凝胶（MCI）和LH-20羟丙基葡聚糖凝胶（Sephadex LH-20）等柱色谱分离方法对乙酸乙酯部位浸膏进行分离纯化,并且利用NMR,MS等各种现代波谱技术以及化合物的理化性质并结合参考文献确定化合物的化学结构。结果：从凉粉草的干燥地上部分的95%乙醇提取物中总共分离得到13个化合物,分别鉴定为熊果酸（ursolic acid,1）,齐墩果酸（oleanic acid,2）,2α,3α-二羟基-12-烯-28-齐墩果酸（2α,3α-dihydroxyolean-12-en-28-oic acid,3）,2α-羟基乌苏酸（corosolic acid,4）,2α,3α,19α-三羟基-12-烯-28-乌苏酸（2α,3α,19α-trihydroxyurs-12-en-28-oic acid,5）,豆甾醇（stigmasterol,6）,β-谷甾醇（β-sitosterol,7）,山柰酚（kaempferol,8）,槲皮素（quercetin,9）,3-吲哚甲酸（3-indoleformic,10）,橙黄胡椒酰胺乙酸酯（aurantiamide acetate,11）,十七烷酸（heptadecanoic acid,12）,十六烷酸（palmitic acid,13）。结论：化合物3~5,7,10~13均为首次从凉粉草以及从凉粉草属植物中分离得到。Objective： To study the chemical constituents from the aerial parts of Mesona chinensis,in order to explore the possibility of the homology between medicine and food. Method： The aerial parts of dry M.chinensis were extracted with 95% ethanol,then the solvent was decompressed and extracted with hexane and ethyl acetate respectively to get hexane fractions and ethyl acetate fractions. The ethyl acetate fractions were isolated and purified by column chromatography with silica gel,MCI and Sephadex LH-20,and their structures were identified by NMR and MS,physicochemical properties and reference literatures. Result： Thirteen compounds were isolated and identified as ursolic acid（ 1）,oleanic acid（ 2）,2α,3α-dihydroxyolean-12-en-28-oic acid（ 3）,corosolic acid（ 4）,2α,3α,19α-trihydroxyurs-12-en-28-oic acid（ 5）,stigmasterol（ 6）,β-sitosterol（ 7）,kaempferol（ 8）,quercetin（ 9）,3-indoleformic（ 10）,aurantiamide acetate（ 11）,heptadecanoic acid（ 12）,palmitic acid（ 13）. Conclusion： Compounds 3-5,7,10-13 were isolated from the plants of M. chinensis and Mesona for the first time.

关 键 词：唇形科 凉粉草 熊果酸 豆甾醇 槲皮素 3-吲哚甲酸 

分 类 号：R284.2[医药卫生—中药学] R22[医药卫生—中医学]