丹皮酚联合三七总皂苷对大鼠糖尿病心肌纤维化的影响  

Effect of Paeonol Combined with Panax notoginseng Saponins on Diabetic Myocardial Fibrosis in Rats

作　　者：贾卓雅 石凯行 范彦芳[1] 李前宽[1] 周晓慧[1] JIA Zhuo-ya, SHI Kai-hang, FAN Yan-fang, LI Qian-kuan, ZHOU Xiao-hui(Chengde Medical University, Chengde 067000, Chin)

机构地区：[1]承德医学院

出　　处：《中国实验方剂学杂志》2018年第6期133-138,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省自然科学基金项目(C2011406009);河北省教育厅重点项目(ZD2016011)

摘　　要：目的：探讨丹皮酚联合三七总皂苷对大鼠糖尿病心肌纤维化的影响,探讨其作用机制。方法：采用高脂高糖饲喂联合腹腔注射链脲佐菌素诱导糖尿病心肌病模型。按照二因素五水平均匀设计法,以心肌组织马松（Masson）染色测定的心肌胶原容积分数为观测指标,采用多元回归分析确定丹皮酚联合三七总皂苷对糖尿病心肌病大鼠最佳组合剂量。运用2×2析因设计试验分析两者之间有无交互作用,设空白组,模型组,丹皮酚（80 mg·kg-1）组,三七总皂苷（100 mg·kg-1）组,丹皮酚（80 mg·kg-1）＋三七总皂苷（100 mg·kg-1）组,二甲双胍（157.5 mg·kg-1）组。检测体质量,心重指数（HWI）,血糖（FBG）,乳酸脱氢酶（LDH）,天门冬氨酸氨基转移酶（AST）,肌酸激酶同工酶MB（CK-MB）的含量,心肌组织中的丙二醛（MDA）,超氧化物歧化酶（SOD）活性变化,观察Masson染色心肌胶原分布。结果：发现丹皮酚（80 mg·kg-1）和三七总皂苷（100 mg·kg-1）联合抗糖尿病心肌病作用最佳并有协同作用。与正常组比较,模型组HWI,FBG,LDH,AST,CK-MB,MDA的含量升高,SOD活性降低,体质量显著下降（P〈0.05）,Masson染色显示心肌胶原纤维明显增高（P〈0.05）。与模型组比较,丹皮酚组,三七总皂苷组,联合用药组的HWI,FBG,LDH,AST,CK-MB,MDA的含量下降,SOD活性升高,体质量显著增高（P〈0.05）,Masson染色显示心肌胶原纤维明显减少（P〈0.05）,而联合用药组与单味用药组比较有显著差异（P〈0.05）。结论：丹皮酚联合三七总皂苷具有协同降低心肌纤维化作用,可能与抗氧化应激作用有关。Objective： To observe the effect of paeonol（ Pae） combined with Panax notoginseng saponins（ PNS） on myocardial fibrosis in rats with diabetes mellitus. Method： Diabetic cardiomyopathy model was induced by high fat and high glucose feeding combined with intraperitoneal injection of streptozotocin（ STZ）.According to the two factors and five levels uniform design method,the myocardial collagen volume fraction measured by Masson staining in myocardial tissue was used as the observation index,and the best combination dose of paeonol and PNS in diabetic cardiomyopathy rats was determined by multiple regression analysis. The 2 × 2 factorial design test was used to analyze the interaction between Pae and PNS. The blank group,model group,Pae group（ 80 mg·kg-1）,PNS group（ 100 mg·kg-1）,Pae（ 80 mg·kg-1） ＋ PNS（ 100 mg·kg-1） group,and metformin（ 157. 5 mg·kg-1） group were established in this study. Then their body weight,heart weight index（ HWI）,blood glucose（ FBG）,lactate dehydrogenase（ LDH）,aspartate aminotransferase（ AST）,creatine kinase isoenzyme MB（ CK-MB）, malondialdehyde（ MDA）, and superoxide dismutase（ SOD） activity in myocardial tissues were detected,and the distribution of myocardial collagen was observed by Masson staining.Result： It was found that the combination of Pae（ 80 mg·kg-1） and PNS（ 100 mg·kg-1） had the best effect and synergistic effect on diabetic cardiomyopathy. As compared with the normal group,the contents of HWI,FBG,LDH,AST,CK-MB and MDA in the model group were increased,the activity of SOD and body weight were decreased significantly（ P〈0. 05）,while the myocardial collagen fiber was significantly increased in Masson staining（ P〈0. 05）. As compared with the model group,the contents of HWI,FBG,LDH,AST,CK-MB and MDA in the model group were decreased. The activity of SOD increased and body weight increased significantly（ P〈0. 05）,and the myocardial collagen fiber was significantly decreased in Masson st

关 键 词：丹皮酚 三七总皂苷 糖尿病 心肌纤维化 

分 类 号：R22[医药卫生—中医基础理论] R24[医药卫生—中医学]