重组人血小板活化因子乙酰水解酶对脑缺血后候选可塑性相关基因15蛋白的表达  

Effect of recombinant human platelet activating factor acetylhydrolase on CPG15 expression after cerebral ischemia

作　　者：王启征 张雄[1] 曹裕民[1] 徐玉玲 唐晓娜 冯淑君[1] 王丽娟[1] Wang Qizheng, Zhang Xiong, Cao Yumin, Xu Yuling, Tang Xiaona, Feng Shuj un, Wang Lijuan(Department of Neurology ,Guangdong People＇s Hospital ,Guangzhou 510080 ,Guangdong Province ,Chin)

机构地区：[1]广东省人民医院神经内科(广东省医学科学院)广东省神经科学研究所,广州510080 [2]汕头大学医学院

出　　处：《中华老年心脑血管病杂志》2018年第3期302-304,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：广东省科技计划(2013B021800193); 广州市科技计划(201510010209)

摘　　要：目的探讨重组人血小板活化因子乙酰水解酶(rPAF-AH)对局灶性脑缺血大鼠缺血区候选可塑性相关基因15(CPG15)蛋白表达的影响。方法选择SD大鼠45只,随机分为假手术组、生理盐水组、rPAF-AH组,每组15只,后2组建立大脑中动脉栓塞模型。选取2、7、14d3个时间点,每个时间点各组分别取5只用于实验。开颅取视交叉后的冠状切块,匀浆提取总蛋白,运用Western blot技术检测CPG15蛋白表达水平。结果生理盐水组和rPAF-AH组2、7、14d缺血区CPG15蛋白表达明显高于假手术组,7d缺血区CPG15蛋白表达最高,且rPAF-AH组7d缺血区CPG15蛋白表达明显高于生理盐水组(1.48±0.04 vs 1.12±0.05,P<0.01)。结论 rPAF-AH能够上调局灶性脑缺血大鼠CPG15蛋白表达,可能在脑缺血的神经重构中发挥积极作用。Objective To study the effect of recombinant human platelet activating factor acetylhydrolase（rPAF-AH）on candidate plasticity-related gene 15（CPG15）expression in focal cerebral ischemia rats.Methods Forty-five SD rats were randomly divided into sham operation group,saline group and rPAF-AH group（15 in each group）.A middle cerebral artery occlusion model of rats was established for the latter two groups.Each group was divided into 2 dgroup,7 dgroup,14 dgroup.Five rats in each of 2 dgroup,7 dgroup,14 dgroup were used in experiment.The total protein was extracted from coronary sections using homogenate.CPG15 protein expression was detected by Western blot.Results The CPG15 protein expression level was significantly higher in saline group and rPAF-AH group than in sham operation group on days 2,7,14 and reached its peak on day 7,and in rPAF-AH group than in saline group on day 7（1.48±0.04 vs 1.12±0.05,P0.01）.Conclusion PAF-AH plays a positive role in neuroplasticity of cerebral ischemia by upregulating the CPG-15 expression in focal cerebral ischemia rats.

关 键 词：血小板活化因子 脑缺血 梗死 大脑中动脉 1-烷基-2-乙酰甘油磷酸胆碱酯酶 

分 类 号：R743.3[医药卫生—神经病学与精神病学]