食管鳞癌组织中TRIM28和p16的表达与临床病理因素的关系  

Expression of TRIM28 and p16 in esophageal squamous cell carcinoma and their clinicopathological significance

作　　者：孙微[1] 刘博[2] 李秀娟[3] 李坤[2] 林媛媛[2] 吕洋[1] 李凤玉[4] SUN Wei, LIU Bo, LI Xiujuan, LI Kun, LIN Yuanyuan, LU Yang, LI Fengyu.(Department of Histology and Embryology, Hebei North University, Zhangjiakou 075000, Chin)

机构地区：[1]河北北方学院组织学与胚胎学教研室,河北张家口075000 [2]河北北方学院附属第一医院病理科,河北张家口075000 [3]河北北方学院病理教研室,河北张家口075000 [4]解放军251医院肿瘤科河北张家口075000

出　　处：《实用医学杂志》2018年第5期760-764,共5页The Journal of Practical Medicine

基　　金：河北省科技厅计划项目(编号：15277707D); 河北省卫生厅科学技术指导项目(编号：ZD20140220); 张家口市科技局计划项目(编号：1712003D)

摘　　要：目的研究三结构域蛋白家族28(TRIM 28)和p16基因在食管鳞癌(ESCC)组织中的表达情况,并观察二者与患者临床病理因素的相关性。方法选取河北北方学院附属第一医院手术切除的ESCC患者肿瘤组织136例,并选取37例距肿瘤边缘5 cm以上的正常食管黏膜组织,用免疫组化SP法及对各个组织中TRIM28、p16蛋白的表达情况进行检测,并采用免疫荧光染色定位二者在ESCC中的表达部分,依据结果对它们与ESCC患者临床病理特征的相关性进行探究。结果 (1)TRIM28与p16在ESCC组织中的阳性率分别为91.2%(124/136)、32.4%(44/136),在正常食管黏膜组织中的阳性率分别为24%(9/37)、57%(21/37),差异均具有统计学意义(P<0.05)。(2)免疫荧光染色显示二者均主要定位于ESCC癌细胞的胞核中。(3)二者的表达情况均与ESCC的TNM分期、浸润程度、淋巴结转移相关。(4)TRIM28与p16在ESCC中的表达呈负相关(r=-0.284,P=0.001)。结论在ESCC发生发展过程中TRIM28及p16均可呈异常表达,协同检测二标记物可辅助ESCC诊断并指导临床治疗。Objective To investigate the expression of TRIM28 and p16 in esophageal squamous cell car- cinoma （ESCC） and explore the possible correlation with them and clinicopathological characteristics. Methods The expression level of TRIM28 and p16 were measured by immunohistochemistry S-P in 136 cases with ESCC and 37 cases with normal esophageal mucosa, selected from the First Affiliated Hospital of Hebei North University De- partment of Pathology. The relationship between them and the clinical-pathological features was also analyzed. The localization of TRIM28 and p16 protein in ESCC was detected by immunofluoreseence. Results （1）The positive rates of TRIM28 and p16 in ESCC were 91.2% and 32.4%, respectively, whereas in normal esophageal mucosa the corresponding rates were 24% and 57%, respectively. （2） Immunofluorescence results showed that TRIM28 and p16 protein were all mainly distributed in the nucleus of ESCC. （3） The abnormal expression of TRIM28 and p16 protein were all related to the invasion depth, TNM staging and lymph node metastasis in ESCC （P 〈 0.05）. （4） The expression of TRIM28 was negatively correlated to the expression of p16 in ESCC （r = -0.284, P = 0.001 ）. Conclusions The abnormal expression of TRIM28 and p16 may have synergistic effect on the initiation and development of ESCC. Co-detection of the expression of them may be useful for diagnosis of ESCC and guiding the clinical therapy.

关 键 词：食管鳞癌 TRIM28 P16 免疫组织化学 荧光免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]